洋葱atp6基因的克隆与系统进化分析  

Cloning and Phylogenetic Analysis of atp6 Gene from Onion

作　　者：王振宝[1,2] 霍雨猛[2] 缪军[2] 高莉敏[2] 刘冰江[2] 杨妍妍[2] 吴雄[2] 程斐[1] 

机构地区：[1]青岛农业大学园艺学院,山东青岛266109 [2]山东省农业科学院蔬菜花卉研究所/山东省设施蔬菜生物学重点实验室/国家蔬菜改良中心山东分中心,山东济南250100

出　　处：《山东农业科学》2014年第1期10-14,共5页Shandong Agricultural Sciences

基　　金：国家自然科学基金项目(31201635;31301786)

摘　　要：细胞质雄性不育(CMS)在作物遗传育种研究中占有重要地位,多种植物的细胞质雄性不育均与线粒体基因结构变异及新嵌合基因的产生有关。为探索线粒体atp6基因与洋葱CMS的关系,以13份不育系和11份保持系为材料克隆洋葱atp6基因编码序列,测序结果表明不同材料atp6基因间存在一个C/A多态性位点,该多态性位点在不育系和保持系间随机出现,而不是不育系与保持系的特征差异。蛋白质分析表明这一多态性位点的变异并未改变atp6蛋白跨膜结构。进一步的蛋白比对分析揭示了atp6蛋白在物种间有一定的保守性,特别是第IV跨膜结构域的Arg在所有物种中是高度保守的,也是H+转运所必需的。基于atp6蛋白序列的系统进化树显示细菌和病毒位于树的基部,其次是真菌、低等藻类以及原生生物,然后高等藻类、苔藓和蕨类,高等绿色开花植物处于树的顶端,单子叶植物处于树的最顶端。Cytoplasm male-sterility （ CMS ） is very important in crop genetic breeding .Usually , this phenomenon is associated with structural variation of mitochondrial gene or formation of novel chimeric gene . In order to explore the relationship between atp6 gene and onion CMS , the coding sequence of atp6 gene was cloned from 13 onion sterile lines and 11 maintainer lines .Sequencing results showed that there was one poly-morphic locus （ C/A） in different onion lines .This polymorphic locus randomly appeared in sterile and main-tainer lines , which was not the characteristic difference between two lines .Protein analysis indicated that the variation of the polymorphic locus didn ’ t change the transmembrane structure of atp 6 protein.Further phylo-genetic analysis revealed that atp 6 protein was conserved among different species .Especially the Arg in the fourth transmembrane domain , which was necessary during the H ＋transport process , was highly conserved . The phylogenetic tree based on atp 6 protein sequence showed that bacteria and viruses were at the base of the tree, followed by fungi , algae and protest , then higher algae , mosses and ferns , higher green flowering plants, especially monocots, were at the top of the tree.

关 键 词：洋葱 雄性不育 atp6基因 系统进化 

分 类 号：Q785[生物学—分子生物学]