虾籽中十种合成色素及其色淀的高效液相色谱测定方法  

Determination of Ten Synthetic Colorants and Their Aluminum Lakes in Shrimp Eggs by High Performance Liquid Chromatography

作　　者：杨昊[1,2] 王骏[1,2] 周莉莉[1,2] 薛霞[1,2] 张卉[1,2] 祝建华[1,2] 于文江[1,2] 

机构地区：[1]山东省产品质量监督检验研究院 [2]国家加工食品质量监督检验中心( 山东),山东济南250103

出　　处：《山东农业科学》2014年第1期110-113,共4页Shandong Agricultural Sciences

基　　金：国家质检总局科技计划项目"食品中30种禁用限用色素的筛查确证技术研究"(2011QK242)

摘　　要：建立了虾籽中柠檬黄、日落黄等10种合成色素及其色淀的高效液相色谱测定方法。样品以氢氧化钠-甲醇溶液提取，加入EDTA络合色淀中的铝离子。采用symmetry C18色谱柱，以甲醇-20 mmol/L醋酸铵为流动相梯度洗脱，二极管阵列检测器测定，外标法定量。10种色素在0．5～20．0 mg/kg范围内线性良好，相关系数均大于0．9999，加标回收率82．4％～107．9％，相对标准偏差0．445％～6．310％，检出限为0．5～1．0 mg/kg。A simultaneous determination method of 10 kinds of synthetic colorants , such as tatrazine and sunset yellow , and their aluminum lakes in shrimp eggs was established by high performance liquid chromatog -raphy.The samples were extracted by methanol -NaOH solution, and the aluminium ions were removed by complexation with EDTA.The extracts were separated with symmetry C18（25 cm ＆#215;4.6 cm ＆#215;5μm）column u-sing methanol-20 mmol/L ammonium acetate as mobile phase .The target compounds were confirmed by di-ode-array detection and quantified by the external standard method .The linear relationships of 10 synthetic colorants were good in the range of 0.5~20.0 mg/kg with correlation coefficients more than 0.9999.The re-covery of standard addition ranged form 82.4%to 107.9%with the relative standard deviation （RSD） in the range of 0.445%~6.310%.And the detection limit was in the range of 0.5~1.0 mg/kg.

关 键 词：虾籽 合成色素 色淀 高效液相色谱 

分 类 号：Q657.72[生物学—生物物理学]