代谢性谷氨酸受体亚型5对骨癌痛小鼠痛行为及脊髓水平炎症反应的影响  

EFFECTS OF METABOTROPIC GLUTAMATE RECEPTOR 5 ON PAIN BEHAVIOR AND SPINAL INFLAMMATION OF MICE WITH BONE CANCER

作　　者：任炳旭[1,2] 季永[1,2] 孙大鹏[1,2] 金琳芳[1,3] 肖寒[1,4] 李国君[1,2] 唐季春[1,2] 惠夏[1,2] 杨定清[1,2] 

机构地区：[1]江南大学附属医院 [2]无锡市第四人民医院麻醉科,无锡214062 [3]无锡市第四人民医院病理科,无锡214062 [4]无锡市第四人民医院中西医结合肿瘤科,无锡214062

出　　处：《中国疼痛医学杂志》2014年第1期30-33,共4页Chinese Journal of Pain Medicine

基　　金：国家自然科学青年基金(81300949);江苏省自然科学青年基金(BK2012102);无锡市医院管理中心医学科技发展基金(YGM1108)

摘　　要：目的:探讨鞘内注射mGluR5拮抗剂MTEP对骨癌痛小鼠热痛觉过敏及脊髓水平IL-1β、IL-6、TNF-α表达的影响。方法:C3H/HeNCrlVr雄性小鼠60只,随机分为:①MTEP+Tumor组:癌痛组从第14 d开始鞘内注射MTEP150nmol,qd,共7次;②生理盐水+Tumor组:以等体积的生理盐水代替MTEP;③MTEP+Sham组:用等剂量MTEP给予假手术组;④生理盐水+Sham组:用等体积的生理盐水给予假手术组;⑤正常组:正常饲养21d。应用热辐射刺激仪测定缩足潜伏期(PWL)。应用Real-time PCR技术检测mRNA表达。结果:各组小鼠术前基础PWL差异无统计学意义(P>0.05)。术后14 d,与正常组相比,骨癌痛组PWL明显降低(P<0.05),假手术组PWL差异无统计学意义(P>0.05)。术后21 d,和正常组相比,MTEP+Sham组、生理盐水+Sham组PWL和脊髓IL-1β、IL-6、TNF-α表达差异均无统计学意义(P>0.05);生理盐水+Tumor组PWL(6.18±1.29)s明显低于正常组(15.91±1.65)s(P<0.05),脊髓IL-1β、IL-6、TNF-α表达则高于正常组;MTEP+Tumor组(9.39±1.94)s PWL高于生理盐水+Tumor组,脊髓IL-1β、IL-6、TNF-α表达则低于生理盐水+Tumor组(P<0.05)。结论:鞘内注射mGluR5拮抗剂抑制脊髓水平表达释放炎症因子IL-1β、IL-6和TNF-α可能是其抗癌痛作用机制之一。Objective： To investigate the effect of intrathecal injection of the mGluR5 antagonist MTEP on bone cancer pain behavor and spinal IL-1β, IL-6, TNF-a expression. Methods： C3H/HeNCrlVr 60 male mice were randomly divided into： O） MTEP＋Tumor group： the tumor mice were treated by intrathecal administration （ once daily on the days 14 - 20 after inoculation of tumor cells） with MTEP 150nmol; physiological saline＋Tumor group： the tumor mice were treated with the same volume of physiological saline; MTEP＋Sham group： the sham mice were treated with MTEP 150nmol; physiological saline＋Sham group： the sham mice were treated with the same volume of physiological saline; normal control group： the mice were given food and water ad libitum.The mice pain behaviors were assessed with the paw withdrawal thermal latency （PWL） at the corresponding time points, then the mice were killed and the samples of spinal cord were used to Real-time PCR. Results： The basic values of PWL had no significant differences amongall groups （P 〉 0.05）. At day 14 after operation, no significant difference （P 〉 0.05） was found in the PWL value between normal control group and the sham operation group. But in tumor group, the PWL value was significantly lower than that of normal control group （P 〈 0.05）. At day 21 after operation, the PWL and the level of spinal IL-1 β IL-6, TNF- a expression had no significant differences （P 〉 0.05） among normal control group, MTEP＋Sham group and physiological saline＋Sham group （P 〉 0.05）; and the PWL （6.18 ±1.29s） in physiological saline＋Tumor group was significantly lower than that of normal control group （15.91±1.65s） （P 〈 0.05）, but the level of IL-113 ~ IL-6, TNF-a expression was higher than that of normal control group; compared with physiological saline＋Tumor group, the PWL （9.39±1.94s） of MTEP＋Tumor group was higher, and the level of spinal IL-1 β, IL-6, TNF- et expression was lower （P 〈 0.05）. Conclusion�

关 键 词：骨癌痛 脊髓 代谢性谷氨酸受体亚型5 白介素-lβ白介素-6 肿瘤坏死因子-A 

分 类 号：R738[医药卫生—肿瘤]