p55PIK上调血管内皮生长因子促进结肠癌细胞血管的生成的作用  

p55PIK promotes angiogenesis of HT29 through upregulating vascular endothelial growth factor

作　　者：杨锐[1] 王桂华[1] 李小兰[1] 邹声泉[1] 胡俊波[1] 

机构地区：[1]430030武汉,华中科技大学同济医学院附属同济医院普外科

出　　处：《中华实验外科杂志》2014年第2期236-238,共3页Chinese Journal of Experimental Surgery

基　　金：973计划资助项目（2009CB521802）;国家自然科学基金资助项目（30973496、81172512、81272278、31000612）;大学长江学者创新研究团队（PCSIRT1131）

摘　　要：目的 观察p55PIK对结肠癌细胞（HT29）的血管内皮生长因子（VEGF）分泌及其对成瘤的影响,探讨肿瘤中p55PIK与血管生成之间的关系.方法 以携带有p55PIK基因的腺病毒感染肿瘤细胞,用实时定量聚合酶链反应（Real-time PCR）检测空白组以及感染空腺病毒和p55PIK腺病毒的肿瘤细胞中VEGF mRNA水平表达差异;用Western blot的方法检测肿瘤细胞VEGF蛋白水平的表达差异;并以感染空腺病毒和感染p55PIK腺病毒的HT29细胞种植裸鼠,观察p55PIK对成瘤性的影响;以移植瘤行免疫组织化学染色,比较p55PIK、VEGF和CD31在组织中表达水平的差异.结果 细胞内过表达p55PIK后,VEGF mRNA表达水平较对照组和空腺病毒组增高2.7倍（P＜0.01）,蛋白表达水平也明显增高（P＜0.01）;成瘤性明显增强,移植瘤的生长速度增快,3组肿瘤重量分别为（0.057 ±0.040）、（0.060±0.030）、（0.225 ±0.070） g（P ＜0.01）.过表达p55PIK组的肿瘤组织中VEGF和CD31的表达水平明显增高.结论 过表达p55PIK的肿瘤细胞成瘤性的增强可能是通过提高VEGF的表达,从而促进肿瘤血管的生成来实现的.Objective To study the effect of p55PIK on colon tumor cells （HT29） secreting vascular endothelial growth factor （VEGF） and tumorgenesis,and the relationship between p55PIK and angiogenesis in tumors.Methods The tumor cells were infected by adenovirus coding p55PIK-GFP,and the mRNA and protein expression level of VEGF in blank control group,empty vector group and p55PIK vector group was detected by using real-time quantitative polymerase chain reaction （Real-time PCR） and Western blotting respectively.In animal study,nude mice xenograft model was made by injecting HT29 cells infected by adenovirus carrying control vector or p55PIK vector respectively.The effect of p55PIK on tumorgenesis was observed,and the expression of p55PIK,VEGF and CD31 in the xenograft of mice was detected by using immunohistochemitry.Results The mRNA level of VEGF in p55PIK vector group was 2.7 times higher in blank control group and empty control group,so was the protein level （P 〈 0.01）.Meanwhile,the tumorigenic ability and growth speed of the xenograft were increased.The average tumor weight in blank control group,empty vector group and p55 PIK vector group was （0.057 ± 0.040） g,（0.060 ±0.030） g and （0.225 ±0.070） g respectively （P 〈0.01）.The expression of VEGF and CD31 in xenograft of mice was significantly increased in p55PIK-overexpression group.Conclusion Overexpressing p55PIK promotes angiogenesis by enhancing VEGF expression in tumor cells,which increases tumorigenesis eventually.

关 键 词：p55PIK 血管内皮生长因子 血管生成 结肠肿瘤 

分 类 号：R735.3[医药卫生—肿瘤]