锌指蛋白217在结直肠癌中的表达及临床意义  

Clinical significance and expression level of zinc finger protein 217 in colorectal cancer

作　　者：王辉[1] 韩亮[1] 张涛[1] 滕莉[1] 王向阳[1] 

机构地区：[1]430014武汉市中心医院胃肠外科

出　　处：《中华实验外科杂志》2014年第2期253-255,共3页Chinese Journal of Experimental Surgery

摘　　要：目的 探讨锌指蛋白217（ZNF217）基因在结直肠癌中的表达特点及其临床意义.方法 应用Western blot检测10例人新鲜结直肠癌和癌旁正常黏膜中ZNF217蛋白表达,并采用免疫组织化学法检测114例结直肠癌组织、癌旁正常黏膜以及淋巴结中ZNF217蛋白表达.结果 ZNF217蛋白在结直肠癌中高表达81例,癌旁正常黏膜中高表达38例,转移淋巴结中高表达51例,癌组织和转移淋巴结ZNF217蛋白表达明显高于正常组织（P＜0.05）,伴有远处转移的癌组织高表达41例,临床Dukes C、D期53例,伴有淋巴结转移的癌组织高表达58例,癌胚抗原（CEA）＞5μg/L的癌组织高表达45例,ZNF217高表达患者和低表达患者平均生存时间分别为（31.8±7.6）个月和（49.7±8.5）个月,ZNF217的表达水平与远处转移、临床Dukes分期、淋巴结转移、CEA以及患者的生存时间密切相关（P＜0.05）.而且,ZNF217表达水平越高,患者死亡风险越高,是结直肠癌独立的预后因素之一（P＜0.05）.结论 ZNF217蛋白表达上升很可能引起结直肠癌的发生、发展、转移,并使患者生存时间缩短.Objective To study the clinical significance and expression level of zinc finger protein 217 （ZNF217） in colorectal cancer.Methods Western blotting was applied to detect the expression level of ZNF217 protein in 10 paired cases of fresh colorectal cancer and para-cancerous normal mucosae,and immunohistochemical method was applied to detect ZNF217 protein in 114 cases of colorectal cancer,paracancerous normal mucosae and metastatic lymph nodes.Results In colorectal cancer,there were 81,38 and 51 cases of high expression of ZNF217 protein in fresh colorectal cancer,normal mucosae and metastatic lymph nodes respectively.The expression level of ZNF217 in para-cancerous mucosae was obviously lower than that in colorectal cancer and metastayiv lymph nodes （P 〈 0.05）.High expression cases of ZNF217 protein with distant metastasis,Dukes stage C and D,lymph node metastasis and carcinoembryonic antigen （CEA） 〉 5 μg/L were respectively 41,53,58 and 45,and survival time of in the patients with ZNF217 high expression and low expression was respectively （31.8 ± 7.6） months and （49.7 ± 8.5）months.The higher the ZNF217 expression was,the higher the dead risk was.The ZNF217 expression level in colorectal cancer was closely related to distant metastasis,Dukes stage,lymph node metastasis,CEA and survival time （P 〈 0.05）.Furthermore,ZNF217 was an independent prognosis factor.Conclusion Up-regulated ZNF217 may well result in occurrence,progress,metastasis of colorectal cancer,and shorter survival time.

关 键 词：锌指蛋白217 结直肠癌 

分 类 号：R735.3[医药卫生—肿瘤]