肝再生磷酸酶-3通过细胞外调节蛋白激酶上调丝氨酸蛋白酶抑制剂E3促进Lovo细胞侵袭  

Phosphatase of regenerating liver-3 promotes Lovo cells invasion by inducing serine protease inhibitor E3 through extracellular signal-regulated kinase

作　　者：许鹤洋[1] 林显敢[1] 罗兴喜[1] 张旸[1] 蓝球生 褚忠华[1] 

机构地区：[1]中山大学孙逸仙纪念医院胃肠外科,广州510120

出　　处：《中华实验外科杂志》2014年第2期259-260,共2页Chinese Journal of Experimental Surgery

基　　金：广东省科技计划资助项目（2012B050600014）;广东省自然科学基金资助项目（S2012010009161）;吴阶平医学基金会临床科研专项基金资助项目（320.6750.12376）

摘　　要：目的 探讨肝再生磷酸酶-3（PRL-3）对丝氨酸蛋白酶抑制剂E3（serpinE3）的影响及机制,以及PRL-3和serpinF3对结肠癌Lovo细胞侵袭性的影响.方法 通过Western blot方法,分别检测已经稳转入PRL-3载体的Lovo-P细胞和对照组Lovo-C细胞中serpinE3的表达水平,Transwell小室检测Lovo细胞侵袭性.再给予细胞外调节蛋白激酶（ERK）特异性抑制剂U0126（10 μmol/L）预处理Lovo-P细胞6h,观察serpinE3表达的变化,检测Lovo-P细胞侵袭性的改变.结果 Western blot检测结果显示在转染人PRL-3的Lovo-P细胞中,serpinE3表达明显上调,Lovo-P细胞侵袭性增强[（378±13）个比（269±15）个,P＜0.05];而当特异性阻断ERK后,Lovo-P细胞中的serpinE3表达下调,并且细胞侵袭性也降低[（21l±9）个比（358±19）个,P＜0.05].结论 PRL-3能够通过ERK诱导serpinE3表达上调的方式,增加Lovo细胞的侵袭性.Objective To investigate the influence and mechanisms of phosphatase of regenerating liver-3 （PRL-3） on serine protease inhibitor E3 （serpinE3）,and their effects on Lovo cells invasion.Methods Western blotting was used to detect the serpinE3 of Lovo-P and Lovo-C cells.Transwell chamber was used to detect the invasion of Lovo-P and Lovo-C cells.The Lovo-P cells were treated with the extracellular signal-regulated kinase （ERK） inhibitor U0126 （10 μmol/L） for 6 h,and the expression of serpinE3 and invasion of Lovo-P cells were examined.Results The expression of serpinE3 was increased in the Lovo-P cells transfected with human PRL-3.Lovo-P cells exhibited stronger invasion ability than Lovo-Ccells （378 ± 13 vs.269 ± 15,P 〈 0.05）.SerpinE3 was abrogated when Lovo-P treated with U0126 and the invasion ability of the cells was decreased either （211-±9 vs.358 ± 19,P 〈0.05）.Conclusion PRL-3 could induce serpinE3 expression by ERK,and then promotes Lovo cells invasion.

关 键 词：肝癌生磷酸酶-3 丝氨酸蛋白酶抑制剂E3 细胞外调节蛋白激酶 

分 类 号：R363[医药卫生—病理学]