假基因Cripto-3 mRNA在人大肠癌组织中的临床意义  

Clinical significance of pseudogene Cripto-3 mRNA in human colorectal carcinoma

作　　者：范钰[1] 徐娟[1] 满昌峰[1] 彭辉勇[1] 祁卫东[2] 蒋鹏程[2] 

机构地区：[1]江苏大学附属人民医院肿瘤研究所,江苏镇江212002 [2]江苏大学附属人民医院普外科,江苏镇江212002

出　　处：《中华实验外科杂志》2014年第2期272-274,共3页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金资助项目（81250035）;江苏省“333工程”科研基金资助项目（BRA2012103）;镇江市社会发展基金资助项目（SH2011031）

摘　　要：目的 探讨假基因Cripto-3（Cr-3）在人大肠癌组织中的临床意义.方法 收集人大肠癌和正常大肠黏膜组织,采用荧光实时定量聚合酶链反应法检测组织中mRNA水平,分析其表达与患者性别、肿瘤发生部位、分化程度、浆膜侵袭、淋巴结转移及Duke＇s分期的相关性,并作统计学处理.结果 在大肠癌组织中Cr-3阳性率为75.0％ （27/36）,在正常大肠黏膜组织中,Cr-3阳性率为0％ （0/36,P＜0.05）.进一步发现Cr-3阳性率分别与浆膜层侵袭、淋巴结转移和Duke＇s分期有关（P＜0.05）;而与患者性别、肿瘤发生部位和分化程度无关（P＞0.05）.结论 假基因Cr-3可能是大肠癌基因诊断和治疗中重要靶点之一.Objective To study the clinical significance of pseudogene Cripto-3 （Cr-3） mRNA in human colorectal carcinoma.Methods After human colorectal normal tissues and colorectal tumoral tissues were collected,the mRNA levels of these tissues were determined by real time polymerase chain reaction.Furthermore,the gender,tumor location,and differentiation degree,and serosal invasion,and lymph node metastasis,and Duke＇s clinical stage were analyzed.Results The positive rates of Cr-3 in colorectal carcinoma was 75% （27/36）,however,in normal colorectal mucosa,the positive rate of Cr-3 was 0%（0/36） （P 〈 0.05）.The positive rates of Cr-3 was closely correlated to the cancer cell invasion,and lymph node metastasis and Duke＇ s stage （P 〈 0.05）,but the positive rates of Cr-3 was not correlated to gender,tumor location and differentiation degree（P 〉 0.05）.Conclusion Cr-3 gene might be one of the important target of gene diagnosis and gene therapy of colorectal cancer.

关 键 词：大肠肿瘤 Cripto-3 

分 类 号：R735.34[医药卫生—肿瘤]