高效液相色谱法测定维药蜀葵花中芦丁和紫云英苷含量  

Efficient determination of rutin and astragalin in Althaea rosea (L.) Gavan from Uyghur medicine

作　　者：勉强辉[1] 郑大成 孙莲[1] 

机构地区：[1]新疆医科大学药学院,乌鲁木齐830011 [2]湖北钟祥市中医医院,湖北钟祥431900

出　　处：《新疆医科大学学报》2014年第2期160-162,共3页Journal of Xinjiang Medical University

基　　金：新疆维吾尔自治区自然科学基金(2013211A055)

摘　　要：目的：建立高效液相色谱法（HPLC 法）测定维药蜀葵花中芦丁和紫云英苷的含量。方法采用YMC-Pack ODS-A C18（4．6 mm＆#215;250 mm，5μm）色谱柱，以流动相 A（0．2％磷酸水溶液）和流动相 B（0．2％磷酸乙腈溶液）进行梯度洗脱，检测波长为360 nm，流速为0．6 mL/min，进样体积10μL。结果芦丁和紫云英苷分别在0．5～6．0μg/mL 和1．0～12．0μg/mL 浓度范围内呈良好线性关系；线性方程分别为 A ＝583．5C -0．623和 A ＝628．3C -0．27，相关系数 r 分别为0．9995和0．9991；芦丁和紫云英苷的平均加样回收率（n ＝6）分别为102．3％和100．8％，RSD 分别为0．89％和0．60％，蜀葵花样品中芦丁和紫云英苷的含量分别为0．30～0．32 mg/g 和0．43～0．46 mg/g。结论本方法简便、准确，重复性好，可用于维药蜀葵花中芦丁和紫云英苷的含量测定，为其质量控制提供参考。Objective HPLC method was used to determinate the content of rutin and astragalin inAlthaea rosea （L .）Gavan from Uyghur Medicine.Methods Analysis was carried out on an YMC-Pack ODS-A C18 （4.6 mm＆#215;250 mm,5 μm）column with 0.2% phosphoric acid in water 0.2% phosphoric acid in acetonitrile as mobile phase in gradient elution.The detective wavelength was 360 nm,flow rate was 0.6 mL/min and injec-tion volume was 10 μL.Results The linear ranges of rutin and astragalin were 0.5-6.0 μg/mL and 1.0-12.0 μg/mL and the linear equation were A =583.5C -0.623 （r =0.999 5）and A =628.3C - 0.270 （r =0.999 1）respectively.The average recoveries were 102.3% and 100.8% with RSD of 0.89% and 0.60% （n=6）respectively.The content of rutin and astragalin in Althaea rosea （L .）Gavan were 0.30-0.32 mg/g （n =3）and 0.43~0.46 mg/g （n =3）respectively.Conclusion The results show that the method is sim-ple,accurate and repeatable,and can be used for the quantitative analysis of rutin and astragalin inAlthaea rosea （L.）Gavan from Uyghur Medicine and can be used as a referent for its quality control.

关 键 词：高效液相色谱法 维药 蜀葵花 芦丁 紫云英苷 含量测定 

分 类 号：R914.1[医药卫生—药物化学]