microRNA-24-2对人骨肉瘤细胞系U-2OS克隆形成能力的影响  

Effect of microRNA-24-2 on colony formation ability of the U-2OS cell line

作　　者：梁德勇[1] 陈升[2] 王野[2] 富伟能[2] 

机构地区：[1]中国医科大学附属第一临床学院骨科,辽宁沈阳110001 [2]中国医科大学医学遗传学教研室,辽宁沈阳110001

出　　处：《解剖科学进展》2014年第1期9-11,共3页Progress of Anatomical Sciences

基　　金：辽宁省教育厅(No.L2010595);国家自然科学基金(No.81172577)资助项目

摘　　要：目的探讨microRNA-24-2对人骨肉瘤U-2OS细胞克隆形成能力的影响。方法应用脂质体法将相关microRNA转染人骨肉瘤细胞系U-2OS。转染12h后,应用实时定量RT-PCR检测各组细胞microRNA-24-2表达水平,应用克隆形成实验检测各组细胞克隆形成能力。结果模拟物转染组U-2OS细胞microRNA-24-2表达水平显著高于对照组,而抑制剂转染组低于对照组(P<0.05)。microRNA-24-2模拟物转染组U-2OS细胞克隆形成能力显著低于对照组,而抑制剂转染组高于对照组(P<0.05)。结论 microRNA-24-2具有抑制骨肉瘤细胞克隆的能力,可望成为骨肉瘤治疗的分子标志物。Objective To explore the effect of microRNA-24-2 on colony formation ability of the U-2OS cell line in vitro. Methods U-2OS cells were randomly allocated into 4 groups： mieroRNA-24-2 mimic group, microRNA- 24-2 inhibitor group, negative control group and normal control group, respectively. The mieroRNAs were transfected into U-2OS cells using LipofectamineTM 2000, and microRNA-24-2 expression level and colony formation ability in the U-2OS cells 48 hours after transfeetion were detected by real-time quantitative RT-PCR （RT-qPCR） and colony formation assays, respectively. Results RT-qPCR results showed that mieroRNA-24-2 level in the U-2OS cells was significantly higher in the microRNA-24-2 mimic group than in controls, but lower in the microRNA-24-2 inhibitor group than in controls, indicating the successive transfection. Colony formation ability was lower in microRNA-24-2 mimic than in controls, but higher in inhibitor group than in the control groups. Conclusion The inhibition of microRNA-24-2 on U- 2OS cells suggests a potential biomarker of microRNA-24-2 in the treatment of osteosarcoma.

关 键 词：microRNA-24—2 骨肉瘤 转染 克隆形成 

分 类 号：R738.1[医药卫生—肿瘤]