microRNA21在大肠癌中的表达及临床意义  

Clinical significance of microRNA21 expression in colorectal cancer

作　　者：陈凯荣[1] 张自强[2] 李勇[3] 陈亚茹[1] 沈志香[4] 

机构地区：[1]启东市人民医院普外科,江苏启东226200 [2]启东市人民医院肛肠外科 [3]启东市人民医院病理科 [4]启东市人民医院内镜室

出　　处：《徐州医学院学报》2013年第12期877-879,共3页Acta Academiae Medicinae Xuzhou

基　　金：启东市科技局资助项目（启科发[2011]40号）

摘　　要：目的 检测microRNA21在大肠癌组织中的表达,并分析其临床意义.方法 采用荧光定量实时聚合酶链反应（RT-PCR）检测60例大肠癌组织标本、20例腺瘤组织及60例正常组织标本中microRNA21表达水平;并分析大肠癌中microRNA21表达量与临床分期之间的关系.结果 microRNA21在大肠癌组织中相对表达水平显著高于腺瘤及正常组织（P＜0.01）,与临床分期及预后有一定的关系.结论 microRNA21在大肠癌组织中表达上调,高表达的microRNA21可能预示着肿瘤的恶性程度及不良预后.Objective To detect the expression of microRNA21 in colorectal carcinoma, and analyze its clinical significance. Methods The expression of microRNA21 in 60 cases of colorectal cancer tissues, 20 cases of adenoma and 20 cases of normal tissues was detected by fluorescence quantitative realtime polymerase chain reaction （RTPCR）. The correlation between microRNA21 expression in colorectal carcinoma and clinical stage of tumor was analyzed. Results The expression level of microRNA21 in colorectal cancer tissues was significantly higher than that in adenoma and norreal tissues （ P 〈 0.01 ）, and The expression of microRNA21 was related with clinical stage of tumor and prognosis. Conclusion Up - regulation of microRNA21 expression in colorectal cancer, the high expression of microRNA21 may indicate a serious illness and poor prognosis.

关 键 词：microRNA21 荧光定量PCR(RT-PCR) 大肠癌 肿瘤分期 

分 类 号：R735.3[医药卫生—肿瘤]