煤矸石地种植草木樨接种从枝菌根真菌的效应  

Effects of AM Fungus on Infection and Growth of Melilotus albus Seedlings in Coal Gangue of Guiyang

作　　者：肖厚军 何佳芳 芶久兰 赵欢 雷正均 颜智华[4] 

机构地区：[1]贵州省农业资源与环境研究所,贵州贵阳550006 [2]农业部贵州耕地保育与农业环境科学观测实验站,贵州贵阳550006 [3]贵州省农业资源环境工程技术研究中心,贵州贵阳550006 [4]贵州煤矿地质工程咨询与地质环境监测中心,贵州贵阳550003

出　　处：《贵州农业科学》2013年第12期119-122,共4页Guizhou Agricultural Sciences

基　　金：贵州省社发攻关项目"贵州煤矿区污染土壤生物修复技术研究与示范"[黔科合SY(2010)3097];贵州省创新能力建设项目"贵州省农业资源高效利用技术创新能力建设"[黔科合院所创能(2011)4002]

摘　　要：为筛选出适宜在煤矸石废弃地生物修复所需的丛枝真菌,以煤矸石粉和耕作土壤作供试基质,进行了草木樨接种不同来源从枝菌根真菌(Arbuscular mycorrhizal fungus,AM)的培养试验,研究AM对草木樨侵染率、促生效应和草木樨对AM的依赖度。结果表明:摩西球囊霉(Glomus mosseae,Gm.)和根内球囊霉(Glomus intraradice,Gi.)菌株均能侵染草木樨根部,耕作土壤上的侵染率显著高于煤矸石粉;不同来源菌株的促生效应有差异,且地上部大于根系;草木樨对不同来源菌株的依赖度也有差异,初步认为草木樨与Gm.-GU(从贵州大学生命科学学院引进的摩西球囊霉)能形成优势共生体。在耕作土壤、煤矸石粉基质上接种Gm.-GU,菌根侵染率分别为69.7%和21.7%,地上部生物量、根系量、全株生物量和菌根依赖度均高于其他菌株。To sift out a few arbuscular mycorrhizal fungus suitable for bioremediation in coal gangue, culture experiments were conducted by inoculating with AM fungus in coal gangue and upland soil to study the effects of AM fungus on infection and growth of M. albus seedlings. The results showed that G. mosseae and G. intraradice could all infect the root of M. albus seedlings. The infection rate was significantly higher in cultivated soil than in coal gangue; The difference of improving the M. albus growth by inoculating with different AM fungus strains was observed, which was bigger in overground part than in the root; The dependence of M. albus to AM fungus was obviously observed. M. albus and Gm.-GU could form dominant symbiont. The infection rates was respectively 69.7% and 21.7% in cultivated soil and coal gangue matrix. The dependence of M. albus to AM fungus of overground biomass, root quantity and complete stool biomass was higher than other strains.

关 键 词：煤矸石 从枝菌根真菌 草木樨 优势共生体 

分 类 号：Q89[生物学] X172[环境科学与工程—环境科学]