1株毒死蜱降解菌的降解特性  

Degradation Characteristics of CB3,a Chlorpyrifos Degrading Bacteria Strain

作　　者：卢洁[1] 曹明星[2] 李晓花[3] 梁同军[3] 鲍海鸥[3] 

机构地区：[1]景德镇学院生化系,江西景德镇333000 [2]江西农业大学,江西南昌330045 [3]中国科学院庐山植物园,江西九江332900

出　　处：《贵州农业科学》2013年第12期127-130,共4页Guizhou Agricultural Sciences

基　　金：江西省科技计划项目(2011BBF60004)

摘　　要：为弄清菌株CB3对毒死蜱的降解特性，采用摇床振荡培养等方法，对从长期受有机磷农药污染的土壤和废水中分离出的1株毒死蜱降解茵的降解特性进行了研究。结果表明：CB3生长和对毒死蜱的降解作用具有一定的同步性，当其生长量最大时，对毒死蜱的降解率达89．6％。初始浓度200～800mg／L时，CB3对毒死蜱的降解率较高；浓度高于800mg／L时，随着初始浓度的增加，降解率逐渐下降；当初始浓度为2000mg／L时，降解率仅32％。接种量低于1mL时，CB3对毒死蜱的降解率随着接种量增加而提高，当接种量在1～2．5mL时，CB3对毒死蜱降解率无明显差异。CB3在PH6．0～7．0，25～35℃条件下对毒死蜱的降解率较高。CB3对有机磷类农药的降解作用具有选择性，利用三唑磷作为碳源时生长良好，利用马拉硫磷和氧乐果作碳源时生长较差，利用辛硫磷和乙酰甲胺磷作为碳源时几乎不生长。The degradation characteristics of CB3, a chlorpyrifos degrading bacteria strain isolated from soil and waste water contaminated by organophosphorus pesticide for long-term was studied. The results showed that there was a certain synchronism between CB3 growth and chlorpyrifos degradation rate and the degradation rate could be up to 89. 6~ when the growth of CB3 was the maximum. The degradation rate reached a higher level when the initial concentration was 200 ~ 800 mg/L but the degradation rate decreased gradually with increase of initial concentration when the concentration was above 800 mg/L. The degradation rate of the initial concentration with 2 000 mg/L was 32 % only. The degradation rate of CB3 to chlorpyrifos rose with increase of inoculation level when the inoculation level was below 1 ml and there was no significant difference in degradation rate when the inoculation level was 1~2.5 ml. The degradation rate of CB3 to chlorpyrifos under pH 6.0～7.0 and 25~35℃ reached a higher level. CB3 grew well on triazophos, grew badly on malathion or omethoate and did not grow on fenthion or acephate, which indicates that CB3 is of the selectivity to the degradation of organophosphorus pesticide.

关 键 词：毒死蜱 降解菌 CB3 降解特性 

分 类 号：S182[农业科学—农业基础科学] X172[环境科学与工程—环境科学]