电针刺激联合骨髓间充质干细胞移植治疗对脑出血大鼠出血灶周边区基质金属蛋白酶9及血清白细胞介素2表达的影响  

Effects of electro-acupuncture stimulation combined with bone marrow mesenchymal stem cells transplantation on the expression of MMP-9 and IL-2 in rats subjected to intracerebral hemorrhage

作　　者：范光碧[1] 陈鸿羽[2] 刘玉[2] 王俊[2] 肖兵[2] 杨朝鲜[3] 

机构地区：[1]泸州医学院解剖教研室,四川省646000 [2]泸州医学院学生科研兴趣小组,四川省646000 [3]泸州医学院神经生物学研究室,四川省646000

出　　处：《中华脑科疾病与康复杂志（电子版）》2013年第5期26-30,共5页Chinese Journal of Brain Diseases and Rehabilitation（Electronic Edition）

基　　金：四川省应用基础研究计划项目(2013JY0075);四川省教育厅重点项目(12ZA077)

摘　　要：目的探讨电针刺激和骨髓间充质干细胞（BMSC）移植联合治疗对脑出血大鼠出血灶周边区基质金属蛋白酶9（MMP-9）及血清白细胞介素2（IL02）表达的影响。方法向成年SD大鼠右侧尾壳核注射肝素和胶原酶诱导脑出血模型，并于术后第3天向出血灶移植BMSC后施以电针刺激（Ea．BMSC组），同时设置BMSC移植组（BMSC组）、电针刺激组（Ea组）和生理盐水注射组（NS组）作为对照。各组分别于治疗后1、3、5、7和14d收集血清和脑组织标本，采用免疫荧光法检测出血灶周边区MMP-9的表达变化，放射免疫法检测血清IL-2的含量。采用单因素方差分析中的LSD法进行统计分析。结果Ea—BMSC组的MMP-9表达在1～7d时低于NS组（t值分别为9．85、5．76、7．05、3．13，P均〈0．05），在全部时间点均低于BMSC组（t值分别为8．91、11．68、11．20、10．11、2．76，P均〈0．05），而在3—14d时则高于Ea组（t值分别为-3．38、-3．98、-5．66、-13．07，P均〈0．05）。Ea．BMSC组的血清IL-2含量在5d和7d时高于Ns组（t值分别为-4．15、-3．81，P均〈0．05），在3d和5d时分别低于和高于BMSC组（t值分别为2．76、-3．69，P均〈0．05），在3d和14d时分别高于和低于Ea组（t值分别为-2．90、5．64，P均〈0．05）。结论电针联合BMSC移植治疗可抑制因单纯BMSC移植治疗所引起的MMP-9表达增高，且较电针组和BMSC组更能使IL-2含量保持在较稳定的水平。Objective To explore the effects of electro-acupuncture（Ea） stimulation combined with bone marrow mesenehymal stem cells （BMSC）transplantation on the expression of matrix metalloproteinase-9 （ MMP-9 ） and interleukin-2 （ IL-2 ） in rats after intracerebral hemorrhage （ ICH ）. Methods ICH rats were replicated by injecting the collagenase and heparin into right caudate putamen. After 3 days, the rats were treated by BMSC transplantation and electro-acupuncture stimulation （Ea-BMSC group）, or dealed with BMSC transplantation（ BMSC group）, electro-acupuncture stimulation （Ea group）and physiological saline injection （ NS group） respectively. The serum and brain tissues of rats were collected at 1,3,5,7 and 14 d after treatment. Immunofluorescence and radioimmunoassay were used to detected expression of MMP-9 and IL-2 respectively. LSD method was performed for statistical analysis. Results Expression of MMP-9 in Ea-BMSC group was lower than that in NS group at 1-7 d（t =9.85,5.76,7.05,3.13,all P 〈0. 05） ,and lower than that in BMSC group at all time points（t = 8.91,11.68,11.20,10. 11,2.76,all P 〈0. 05） ,but higher than that of Ea group .at 3-14 d （ t = - 3.38, - 3.98, - 5.66, - 13.07, all P 〈 0. 05 ）. The content of IL-2 inserum in Ea-BMSC group was higher than that of NS group at 5 d and 7 d（t = -4. 15, -3.81,all P 〈 0. 05 ）, and lower and higher than that in BMSC group at 3 d and 5 d respectively （ t = 2. 76, - 3.69, all P 〈 0. 05） ,and higher and lower than that in Ea group at 3 d and 14 d respectively（t = -2.90,5.64,all P 〈 0. 05）. Conclusions Electro-acupuncture combined with BMSC transplantation can inhibit MMP-9 increase caused by simple BMSC transplantation, and helps to maintain the content of IL-2 at a relatively stable level compared to Ea group and BMSC group.

关 键 词：脑出血 电针 白细胞介素2 基质金属蛋白酶9 骨髓间充质干细胞 

分 类 号：R743.34[医药卫生—神经病学与精神病学]