Neural progenitor cells from human induced pluripotent stem cells generated less autogenous immune response  

作　　者：HUANG Ke LIU PengFei LI Xiang CHEN ShuBin WANG LiHui QIN Li SU ZhengHui HUANG WenHao LIU JuLi JIA Bei LIU Jie CAI JingLei PEI DuanQing PAN GuangJin 

机构地区：[1]Key Laboratory of Regenerative Biology, Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health,Chinese Academy of Sciences [2]Department of Regeneration Medicine, School of Pharmaceutical Science, Jilin University [3]Laboratory of Pathogen Biology, State Key Laboratory of Respiratory Disease [4]Department of Obstetrics and Gynecology, Nanfang Hospital, Southern Medical University

出　　处：《Science China(Life Sciences)》2014年第2期162-170,共9页中国科学（生命科学英文版）

基　　金：supported by the National Basic Research Program of China,Ministry of Science and Technology(2011CB965204,2012CB966802);the National Natural Science Foundation of China(31000402);the Strategic Priority Research Program of the Chinese Academy of Sciences(XDA01020401,XDA-01020202);the Ministry of Science and Technology International Technology Cooperation Program(2012DFH30050);the National Science&Technology Major Special Project on Major New Drug Innovation(2011ZX09102-010-01);the Development and Technology Innovation for Equipment Functional Development Project of Chinese Academy of Sciences(yg2011082,yg2011083)

摘　　要：The breakthrough development of induced pluripotent stem cells(iPSCs)raises the prospect of patient-specific treatment for many diseases through the replacement of affected cells.However,whether iPSC-derived functional cell lineages generate a deleterious immune response upon auto-transplantation remains unclear.In this study,we differentiated five human iPSC lines from skin fibroblasts and urine cells into neural progenitor cells(NPCs)and analyzed their immunogenicity.Through co-culture with autogenous peripheral blood mononuclear cells(PBMCs),we showed that both somatic cells and iPSC-derived NPCs do not stimulate significant autogenous PBMC proliferation.However,a significant immune reaction was detected when these cells were co-cultured with allogenous PBMCs.Furthermore,no significant expression of perforin or granzyme B was detected following stimulation of autogenous immune effector cells(CD3+CD8 T cells,CD3+CD8+T cells or CD3 CD56+NK cells)by NPCs in both PBMC and T cell co-culture systems.These results suggest that human iPSC-derived NPCs may not initiate an immune response in autogenous transplants,and thus set a base for further preclinical evaluation of human iPSCs.The breakthrough development of induced pluripotent stem cells（iPSCs）raises the prospect of patient-specific treatment for many diseases through the replacement of affected cells.However,whether iPSC-derived functional cell lineages generate a deleterious immune response upon auto-transplantation remains unclear.In this study,we differentiated five human iPSC lines from skin fibroblasts and urine cells into neural progenitor cells（NPCs）and analyzed their immunogenicity.Through co-culture with autogenous peripheral blood mononuclear cells（PBMCs）,we showed that both somatic cells and iPSC-derived NPCs do not stimulate significant autogenous PBMC proliferation.However,a significant immune reaction was detected when these cells were co-cultured with allogenous PBMCs.Furthermore,no significant expression of perforin or granzyme B was detected following stimulation of autogenous immune effector cells（CD3＋CD8 T cells,CD3＋CD8＋T cells or CD3 CD56＋NK cells）by NPCs in both PBMC and T cell co-culture systems.These results suggest that human iPSC-derived NPCs may not initiate an immune response in autogenous transplants,and thus set a base for further preclinical evaluation of human iPSCs.

关 键 词：induced pluripotent stem cells IMMUNOGENICITY iPSC-derived neural progenitor cells 

分 类 号：R392.1[医药卫生—免疫学]