Inhibition of BCNU-Resistance in Glioma Cell Lines by Small Interfering Rna Targeting to Interleukin-8 Gene  

作　　者：Si-Ming Xie Hui GUO Yong-Rong Yan Xue-Yun Zhong 

机构地区：[1]School of Medicine, Jinan University, Guangzhou City, Guangdong 510632, China [2]Postdoctoral station of Clinical Medicine, Jinan University, Guangzhou, Guangdong 510632, China

出　　处：《Journal of US-China Medical Science》2013年第5期162-169,共8页美中医学（英文版）

摘　　要：It had been found that interleukin-8 （IL-8） was associated with drug resistance. We previously demonstrated that the resistance to 1, 3-bis （2-chloroethyl）-1-nitrosourea （BCNU） of glioma cell line SWOZI was much higher than that of cell line SOWZ2, which were both cloned from the same parental glioma cell line SWO38. In this study, IL-8 was found to be upregulated both in SWOZ1 and SWOZ2-BCNU, a BCNU-resistant glioma cell line. To further investigate the function of IL-8, the BCNU-resistant cell lines SWOZ1 and SWOZ2-BCNU were treated with siRNAs targeting IL-8. The results of quantitative RT-PCR showed that a decreased level of IL-8 mRNA expression in SWOZI and SWOZ2-BCNU for more than 90% compared to negative control and was confirmed by western blot assay （P 〈 0.05） after treated by siRNAs targeting IL-8. Subsequently, the cytotoxicity of BCNU to these cell lines was detected using the cell counting kit-8 assay. As a result, the BCNU resistance was reversed for about 50% both in these two cell lines （P 〈 0.05）. Our data demonstrated that inhibition of IL-8 with specific siRNAs can reverse the BCNU resistant phenotype in glioma cell lines, indicating that IL-8 may play an important role in BCNU-resistance in glioma.

关 键 词：GLIOMA IL-8 siRNA drug resistance BCNU. 

分 类 号：Q522[生物学—生物化学] TV736[水利工程—水利水电工程]