山东地区精子生成障碍与雄激素受体基因（CAG）n多态性的关联分析  

Correlation between androgen receptor gene （CAG）n polymorphism and spermatogenesis deifciency in males of Shangdong

作　　者：刘红英[1] 杨利丽[1] 王刚[1] 

机构地区：[1]潍坊医学院遗传学教研室,潍坊261053

出　　处：《中国男科学杂志》2013年第11期23-26,共4页Chinese Journal of Andrology

基　　金：国家人口与计划委员会C1类项目（2011-C1-89）

摘　　要：目的：探讨雄激素受体（AR）基因（CAG）n多态性与山东地区精子生成障碍发生的相关性。方法选择101例男性不育症患者为研究对象，按照精子密度分为轻度少精子症、重度少精子症、无精子症3组；30例正常男性为对照组。采用PCR与DNA序列分析方法进行AR基因外显子1 CAG序列重复次数的测定。比较各组指标之间的差异，分析该多态性与精子生成障碍之间的关系。结果3个患者组AR基因CAG重复次数分别为26.3±0.9、27.9±2.3、30.8±1.1，均明显高于对照组（22.7±1.6，P＜0.05），且随着精子密度的降低，CAG重复次数逐渐增加（r=-0.670, P=0.037）。结论 AR基因CAG序列重复次数在山东地区男性不育患者中呈多态分布，且与精子生成障碍的发生密切相关。Objective To study the relationship of androgen receptor gene （CAG）n polymorphism distribution and spermatogenesis deficiency in males of Shandong. Methods Total of 101 patients with male infertility and 30 normal males were enrolled in the study . All patients were divided into wild oligospermatism group, severe oligospermatism group and azoospermia group. The length of their AR gene CAG repeat sequence were detected by PCR and DNA sequencing. The data was comparatively analyzed to determine their relationship. Results The number of CAG repeats in patients group were 26.3±0.9, 27.9±2.3, 30.6±1.4 respectively which was significantly higher than that of the control group（22.7±1.6, P＆lt;0.05）. The number of CAG repeats accordingly increased as the sperm count decreased （r=-0.670, P=0.037）. Conclusion The polymorphism of AR gene CAG repeats may be closely associated with the spermatogenesis deficiency in males of Shandong.

关 键 词：精子发生 雄激素受体基因 多态性 

分 类 号：R698.2[医药卫生—泌尿科学]