乳腺癌中NF-κB蛋白表达及其与TNF-α相关性研究  

Expression of Nuclear Factor Kappa B and Correlations with Tumor Necrosis Factor-α in Breast Cancer

作　　者：杨桂[1] 喻明霞[1] 范维[1] 徐婷[1] 陈浩[1] 

机构地区：[1]武汉大学中南医院检验科&基因检测中心,湖北武汉430071

出　　处：《现代生物医学进展》2013年第30期5860-5863,共4页Progress in Modern Biomedicine

基　　金：国家自然科学基金项目(30901308);高等学校博士点学科点专项科研基金(新教师基金)(20090141120025);武汉市青年科技晨光计划项目(20105023106)

摘　　要：目的:研究乳腺癌中细胞核因子-κB(nuclear factor-kappa B,NF-κB)的表达及其与肿瘤坏死因子-α(tumor necrosis factorα,TNF-α)的相关性。方法:采用免疫组织化学的方法检测67例原发乳腺癌组织中NF-κB和TNF-α的表达,检测NF-κB在癌旁正常组织、乳腺增生和乳腺癌中的表达情况,并分析NF-κB的表达与乳腺癌临床病理特征的关系以及NF-κB与TNF-α的相关性。结果:1)NF-κB在癌旁正常组织、乳腺增生和乳腺癌中表达的阳性率分别为20%(3/15)、33.3%(5/15)、79.1%(53/67);2)NF-κB的表达与年龄、肿瘤大小、组织学类型均无相关性(P>0.05),与淋巴转移的有无及组织学分期有显著相关性(P<0.05);3)NF-κB的表达与TNF-α呈显著正相关(P<0.05)。结论:乳腺癌中NF-κB的表达显著增加且与其淋巴转移的有无及肿瘤组织学分期有显著相关性,与TNF-α呈显著正相关,对乳腺癌良恶性判断、诊断、靶向治疗及预后提供重要参考依据。Objective： To determine the expression of nuclear factor kappa B（NF-κB） in breast cancer and its correlation with tumor necrosis factor-α（TNF-α）. Methods： Immunohistochemistry was used to detect the expressions of NF-κB and TNF-α in 67cases of primary breast cancer, and analyzed the association of NF-κB with Clinical and pathological features of breast cancer and TNF-α.Results： 1） The positive expression rate of NF-κB in Paracancerous normal tissue, breast hyperplasia and breast cancer were 20%（3/15）、33.3%（5/15）, 79.1%（53/67） respectively; 2）There were no significant association of NF-κB with age、tumor size, histological type（P0.05）, but significant correlation with presence or absence of lymphnode metastasis and Histologic staging（P0.05）; 3）The expression of NF-κB had a positive correlation with TNF-α. Conclusion： The expression of NF-κB in breast cancer increased notably and had a significant correlation with presence or absence of lymph node metastasis and Histologic staging, and had a positive correlation with TNF-α, so it provide an important reference in diagnosis, targeted therapy and prognosis of breast cancer Malignant and Benign of breast cancer.

关 键 词：乳腺癌 NF-ΚB TNF-Α 

分 类 号：R737.9[医药卫生—肿瘤]