胃癌中TAMs与血管生成及转移相关性的分析  

Analysis of the Relationship of TAMs and Tumor Angiogenesis and Metastasis in Gastric Cancer

作　　者：韩伟[1] 王正彩[2] 周敏[1] 顾云鹤[1] 胡成乙[2] 张淑君[2] 吴红梅[1] 齐蕾[3] 周秋锋[1] 王瑞芬[4] 管雯斌[4] 王立峰[4] 

机构地区：[1]哈尔滨医科大学附属第一医院病理科,黑龙江哈尔滨150001 [2]哈尔滨医科大学附属第四医院病理科,黑龙江哈尔滨150001 [3]河北省邢台市人民医院,河北邢台054031 [4]上海交通大学医学院附属新华医院病理科,上海200092

出　　处：《现代生物医学进展》2013年第30期5881-5884,共4页Progress in Modern Biomedicine

基　　金：黑龙江省教育厅科学技术研究项目(12511191)

摘　　要：目的:研究胃癌中肿瘤相关巨噬细胞(tumor associated macrophages,TAMs)的表达及其与肿瘤血管生成的关系,进而分析其与转移的相关性。方法:利用免疫组化双染方法同时标记TAMs(抗CD68抗体)和内皮细胞(抗CD34抗体),检测90例胃癌中TAMs、微血管密度(microvessel density,MVD)。结果:①TAMs与胃癌的大小、淋巴结转移、远处转移呈显著正相关(P均<0.05);②MVD与胃癌的远处转移呈显著正相关(P均<0.05);③在胃癌组织中TAMs与MVD的表达呈显著的正相关(r=0.325,P=0.002)。结论:TAMs可能通过促进胃癌血管的生成,进而促进胃癌细胞发生血管内渗,最终实现胃癌细胞的转移。Objective： To investigate the expression of tumor associated macrophages（TAMs） and their relationship between TAMs, tumor angiogenesis and metastasis in gastric cancer. Methods： The expression of TAMs and microvessel density（MVD） in 90 case of Paraffin-embedded gastric cancer samples were stained using double-immunostaining method allowing simultaneous identification of macrophages and endothelial cells. Results： ① TAMs density had a positive relationship with the tumor size, lymph node metastasis,distant metastasis（P0.05）;②MVD had a positive correlation with distant metastasis（P 0.05）;③Positive correlation of TAMs and MVD was found in gastric carcinoma（r=0.325,P=0.002）. Conclusions： TAMs could assist tumor cell intravasation and metastasis through promoting angiogenesis in gastric cancer.

关 键 词：胃癌 肿瘤相关巨噬细胞 血管生成 微血管密度 

分 类 号：R735.2[医药卫生—肿瘤]