过表达PPAR γ1基因对大鼠心肌缺血再灌注后心梗面积和炎症反应的影响  

Effects of over-expression of PPAR gamma 1 gene on the changes of myocardial infract areas and inflammatory response induced by ischemia reperfusion

作　　者：陈文雁[1] 魏来[1] 孔高茵[1] 

机构地区：[1]湖南省人民医院麻醉科,湖南长沙410005

出　　处：《中国现代医学杂志》2013年第34期30-34,共5页China Journal of Modern Medicine

摘　　要：目的研究过表达过氧化物酶体增殖物激活受体γ1(PPARγ1)基因对心肌缺血-再灌注后梗死面积、形态学、选择素E和选择素P mRNA表达的影响。方法 30只SD大鼠随机分为3组(n=10),包括假手术组(Sham组)、心肌缺血-再灌注损伤组(MIRI组)、过表达PPARγ1组(PPARγ1组)。Sham组和MIRI组经冠脉转染携带绿色荧光蛋白的腺病毒载体(Ad-EGFP)至心肌组织,PPARγ1组转染携带PPARγ1基因的腺病毒载体(Ad-PPARγ1)。待目的基因转染3 d后,Sham组仅开胸,不阻断左前降支,MIRI组和PPARγ1组建立心肌缺血-再灌注损伤模型,结扎冠脉左前降支30 min,开放120 min后,检测梗死面积、形态学、选择素E和选择素P mRNA的表达情况。结果再灌注末,MIRI组和PPARγ1组心梗死面积、选择素E和选择素P mRNA的表达明显上调,PPARγ1组低于MIRI组(P<0.05),MIRI组和PPARγ1组缺血面积百分比无明显差异(P>0.05)。光镜下观察MIRI组和PPARγ1组炎症反应强于Sham组,MIRI组强于PPARγ1组。结论大鼠心肌过表达PPARγ1基因能够改善心肌缺血-再灌注损伤,减少心梗面积,抑制炎症反应,其机制可能与抑制选择素E和选择素P mRNA的表达上调有关。[ Objective ] To explore the effects of overexpression of PPAR gamma 1 gene on the changes of infract areas, morphosis and inflammatory response induced by myocardial ischemia reperfusion injury. [Method] Thirty SD rats were randomly divided into three groups （n =10）： including sham operation group （Sham group）, myocardial isehemia-reperfusion injury group （MIRI group） and PPARγ1 gene overexpression group （PPARγ1 group）. Myocar- dial tissues were transfected with recombinant adenovirus vector mediated enhanced green fluorescent protein （Ad- EGFP） via coronary artery in Sham group and MIRI group, and myocardial tissues were transfected with recombi- nant adenovirus vector mediated human PPARγ1 gene （Ad-PPARγ1） in PPARyl group. Operations were performed three days after target gene transference. In sham operation group, the rats only underwent open-chest surgery, but not ligation. The left anterior descending coronary artery of rats in the MIRI group and PPARγ1 group was ligated for 30 rain. The myocardial infract areas, morphosis, E-selectin and P-selectin were detected 120 rain later. [Resuits ] At the end of reperfusion, the infract areas, morphosis changes, mRNA expression of E-selectin and P-se- lectin increased in MIRI group and PPARγ1 group. Meanwhile, these results in PPARyl group were significantly less than the MIRI group （P 〈0.05）. There were no significant differences in the percentage of myocardial ischemicarea between two groups （P 〈0.05）. The inflammatory response in MIRI group and PPARγ1 group was stronger than that of Sham group under light microscope, and that in PPARγ1 group was stronger than MIRI group. [ Conclusion] Overexpression of PPARγ1 gene in rat myocardial tissue could improve myocardial ischemia-reperfusion injury, mainly for the myocardial infarction area reduction and inflammatory response inhibition. The mechanism may be re- lated to its inhibition of P-selectin and E-selectin mRNA expression up-regulation.

关 键 词：心肌缺血-再灌注损伤 过氧化物酶体增殖物激活受体γ1 心梗 炎症反应 选择素 

分 类 号：R-332[医药卫生]