神经病理性疼痛大鼠触液核BKca-α表达降低  

THE DECREASE OF BKca-α EXPRESSION IN CSF-CONTACTING NUCLEUS IN NEUROPATHIC PAIN RATS

作　　者：倪林花 张励才[1] 

机构地区：[1]徐州医学院江苏省麻醉学重点实验室,徐州221002

出　　处：《中国疼痛医学杂志》2013年第11期664-669,共6页Chinese Journal of Pain Medicine

基　　金：国家自然科学基金资助(30871307)

摘　　要：目的:观察正常大鼠触液核内BKca-α分布及其在神经病理性疼痛过程中的表达变化,探讨触液核在神经病理性疼痛中的作用。方法:采用霍乱毒素B结合辣根过氧化酶(CB-HRP)和BKca-α免疫荧光双重标记技术,观测大鼠触液核中BKca-α在正常及慢性坐骨神经压迫损伤(chronic constriction injury,CCI)大鼠的表达变化。结果:正常和CCI大鼠触液核内存在BKca-α;在CCI大鼠,大鼠热痛觉及机械痛觉阈值下降,且触液核内BKca-α表达的数目也相应减少,和对照组比较有显著差异。结论:触液核BKca-α的降低可能参与神经病理性疼痛。Objective ： To observe the expression of BKca-α in CSF-contacting nucleus（CSF-CN） of normal rats and changes in it＇s expression during the process of neuropathic pain,and to discuss the role of BKca-αin CSF-CN in neuropathic pain.Methods： The CB-HRP/BKca-α immunofluorescence double-labeling method was used to observe the expression of BKca-α in CSF-CN in normal and chronic constriction injury（CCI） rats.Results： BKca-α was found in CSF-CN in normal and CCI rats.The thresholds of thermal and mechanical pain were remarkably decreased in CCI rats.Compared with sham rats,the expression of BKca-α in CSF-CN was decreased in CCI rats.Conclusion： The decrease of BKca-α in CSF-CN may play an important role in neuropathic pain.

关 键 词：触液核 BKca-α 神经病理性疼痛 

分 类 号：R741[医药卫生—神经病学与精神病学]