沉默GST-π基因表达对EC9706/cDDP细胞顺铂耐药性的影响  

Effect of GST-π expression and mechanisms of reversing drug-resistant to EC9706 / cDDP of esophageal cancer cells

作　　者：唐悦[1] 轩小燕[1] 柳璐璐[1] 李敏[1] 

机构地区：[1]郑州大学基础医学院微生物学与免疫学教研室,郑州450001

出　　处：《郑州大学学报（医学版）》2014年第1期4-8,共5页Journal of Zhengzhou University(Medical Sciences)

摘　　要：目的:观察沉默谷胱甘肽S转移酶-π(GST-π)基因的表达对食管鳞状细胞癌顺铂耐药细胞系EC9706/cDDP细胞耐药性的影响。方法:构建靶向GST-π基因的siRNA重组慢病毒GSTsi1、GSTsi2和无义对照GSTsiC,转染EC9706/cDDP细胞。通过RT-PCR及Western blot法检测GSTsi1、GSTsi2和GSTsiC感染前后EC9706/cDDP细胞中GST-πmRNA和蛋白的表达;MTT法检测感染GSTsi2、GSTsiC与未感染的EC9706/cDDP细胞对顺铂敏感性的变化。结果:感染GSTsi1、GSTsi2后EC9706/cDDP细胞GST-πmRNA的表达下调(F=3.490,P<0.001),其蛋白表达也减弱。感染GSTsi2的EC9706/cDDP细胞对顺铂的耐药指数较感染GSTsiC和未感染的EC9706/cDDP细胞降低(F=50.510,P<0.001)。结论:沉默GST-π基因的表达可降低EC9706/cDDP细胞的顺铂耐药性。Aim ： To observe the effects of silencing the expression of GST-πou the drug-resistance of esophageal car- cinoma cells. Methods： The lentivirus of siRNA targeting to GST-π was constructed. EC9706/cDDP cells were infected by the lentivirus named GSTsil, GSTsi2. The mRN＆ and protein expressions of GST-π before and after infection were measured by RT-PCR and Western blot. Cells were divided into four groups： cells infected with GSTsi2, GSTsiC, untreat- ed EC9706/cDDP and EC9706. Drug sensitivity to cDDP was determined by MTT. Results： The expression level of GST-π mRNA in ceils infected by GSTsil and GSTsi2 were lower than those in control ceils（ F = 3. 490 ,P 〈 0. 001 ） , and the ex- pression of GST-π protein was also decreased. Compared with untreated EC9？06/cDDP and EC9706/cDDP infected by GSTsiC ,the resistance index of EC9706/cDDP infected by GSTsi2 decreased （F = 50. 510 ,P 〈 0. 001 ）. Conclusion： Silencing the expression of GST-π by RNAi can partly reverse the drug resistance of EC9706/cDDP.

关 键 词：EC9706 cDDP细胞 耐药 谷胱甘肽S转移酶-Π RNA干扰 

分 类 号：R735.1[医药卫生—肿瘤]