沙颍河铅污染对儿童GH-IGF-1轴和体格发育的影响  

Influence of lead from Shaying River on children's GH-IGF-1 axis and physical development

作　　者：罗毅鑫[1,2] 黄祺[2] 唐春宇[1] 程学敏[1] 高亚琳[2] 陈虹钖 黄楠[2] 李桉琪[2] 薛玉堂 左其亭[4] 巴月 崔留欣 

机构地区：[1]郑州大学公共卫生学院环境卫生学教研室,郑州450001 [2]郑州大学临床医学系,郑州450052 [3]沈丘县疾病预防控制中心,沈丘466300 [4]郑州大学水利与环境学院,郑州450001

出　　处：《郑州大学学报（医学版）》2014年第1期25-28,共4页Journal of Zhengzhou University(Medical Sciences)

基　　金：国家重大水专项子课题2009ZX07210-006-3-3;全国大学生创新创业训练计划项目121045961

摘　　要：目的：探讨沙颍河铅污染现状及其对儿童生长激素-胰岛素样生长因子-1（ GH-IGF-1）轴和体格发育的影响。方法：随机选择淮河沙颍河流域S县两个村庄作为调查点（依距河岸距离远近分别定为对照区和污染区）；原子吸收分光光度法检测河水和调查点饮用水及土壤中的铅含量。整群抽取两村庄小学8～13岁在校学生作为研究对象，伏安极谱法测定儿童血清铅含量；ELISA法检测儿童血清胰岛素样生长因子-1（ IGF-1）、胰岛素样生长因子结合蛋白3（IGFBP3）、生长激素（GH）和生长激素结合蛋白（GHBP）水平；电子体重计、身高坐高计、皮褶厚度计及Gulick卷尺分别测量体重、身高、皮褶厚度和胸围。结果：河水3个采样断面铅浓度均超过国家地表水水质标准Ⅴ级。污染区饮用水及土壤铅含量均高于对照区（ t＝2．663和2．300，P＜0．05）。污染区儿童血清铅含量高于对照区儿童（t＝3．227，P＝0．002）。污染区男孩身高、体重、皮褶厚度、胸围与对照区比较差异均无统计学意义（P均＞0．05）。两区女孩身高、体重和胸围差异均无统计学意义（P均＞0．05），但污染区女孩皮褶厚度高于对照区（t＝3．036，P＝0．003）。结论：沙颍河流域的饮用水中铅含量明显升高，污染区儿童血清GH-IGF-1轴相关因子水平受到影响。Aim：To investigate the effect of lead from Shaying River on children＆#39;s growth hormone , insulin-like growth factor-1 （GH-IGF-1） axis and physical development .Methods：Two villages in S county were selected randomly as pollu-ted area and control area according to the distance from the Shaying River Basin .Lead levels were measured both in the riv-er water and the drinking water .Children aged 8 to 13 years studying in the village primary school were recruited by cluster sampling.The concentration of lead in serum was measured by polarographic method .Serum insulin-like growth factor-1 （IGF-1）, insulin-like growth factor binding protein 3 （IGFBP3）, growth hormone （GH）, and growth hormone binding proteins （GHBP） were measured by ELISA.The indexes including body weight , height,skinfold thickness and chest-circ-umference were measured .Results：The concentrations of lead exceeded classⅤstandards of the surface water quality in section of the river .Compared with control area , the concentration of lead both in drinking water and soil was significantly higher than those in the polluted area （t=2.663 and 2.300,P＆lt;0.05）.Serum lead level in children from the polluted are-a was higher than that from control area （t=3.227,P=0.002）.Height, weight, skinfold thickness and chest-circumfer-ence did not show significant differences between the boys of the two groups （P＆gt;0.05）.Only skinfold thickness had sig-nificant difference between the girls of the two groups （t=3.036,P=0.003）.Conclusion：The concentration of lead in drinking water of the Shaying River Basin has increased significantly .The GH-IGF-1 axis of children in the polluted areas has been affected .

关 键 词：铅 儿童 生长激素 生长激素结合蛋白 胰岛素样生长因子-1 胰岛素样生长因子结合蛋白3 INSULIN-LIKE GROWTH factor-1 INSULIN-LIKE GROWTH factor binding protein 3 

分 类 号：R123[医药卫生—环境卫生学]