放线菌SIIA243对维生素D_3的羟基化研究  

Study on hydroxylation of vitamin D_3 by an actinomycetes SIIA 243

作　　者：曾志刚[1] 罗红兵[1] 翟龙飞[1] 徐欣[1] 俞岩青 黄海[1] 

机构地区：[1]成都大学四川抗菌素工业研究所,成都610052

出　　处：《中国抗生素杂志》2014年第2期114-117,共4页Chinese Journal of Antibiotics

基　　金：四川省科技厅技术市场及成果交易计划研究项目(项目编号2008CO0002);科技部"十二五"重大新药创制专项子项目(项目编号2011ZX09401-403-9)

摘　　要：目的研究放线菌SIIA243对维生素D3的羟基化作用。方法将VD3加入到不同菌株的培养液中,通过高效液相色谱检测培养液中VD3的转化产物以确定目标菌株,然后在50升发酵罐中培养目标菌株,添加维生素D3,发酵完成后对转化产物进行分离纯化和结构鉴定。结果发现菌株SIIA243转化产物的高效液相图谱中,转化产物1的保留时间与25OHVD3基本相同,转化产物2的保留时间与1α,25(OH)2VD3基本相同;经紫外光谱、质谱和核磁共振谱分析,纯化后得到的两个转化产物分别鉴定为25OHVD3和1α,25(OH)2VD3。结论菌株SIIA243能够将维生素D3转化为25OHVD3和1α,25(OH)2VD3。Objective Study on hydroxylation of vitamin D3 by an actinomycetes SIIA243. Methods Vitamin D3 was incubated with growing culture of different strains, the transformation products from vitamin D3 were detected by HPLC in order to find the target strain.Then, the target strain was cultured in a 50L fermentor and vitamin D3 was added. After completion of fermention, the transformation products were purled and the structures were elucidated. Results According to the HPLC chromatogram of transformation products of strain SIIA243, the retention time of the transformation product 1 was approximately the same as the retention time of 25OHVD3, the retention time of the transformation product 2 was approximately the same as the retention time of 1α, 25（OH）EVD3, two purified transformation products were separately elucidated as 25OHVD3 and 1α, 25（OH）EVD3 by means of spectral analysis including UV, MS and NMR. Conclusion Strain SIIA243 was able to convert vitamin D3 to 25OHVD3 and 1α, 25（OH）2D3.

关 键 词：VD3 25OHVD3 1α 25(OH)2VD3 羟基化 

分 类 号：Q56[生物学—生物化学]