快速溶剂萃取提取白藜芦醇和白藜芦醇苷工艺的响应面优化  

Optimization of Accelerated Solvent Extraction of Resveratrol and Polydatin Using Response Surface Methodology

作　　者：曹静亚[1,2] 迟晓峰[1,2] 陈涛[1,2] 董琦[1,2] 谭亮[1,2] 胡风祖[1,2] 

机构地区：[1]中国科学院藏药研究重点实验室 [2]中国科学院西北高原生物研究所,西宁810008

出　　处：《天然产物研究与开发》2014年第1期136-141,共6页Natural Product Research and Development

基　　金：中国科学院知识创新工程重要方向项目(KSCX2-EW-J-26)

摘　　要：以白藜芦醇和白藜芦醇苷的总量为指标,考察了超声、加热回流、快速溶剂萃取(ASE)三种提取方法对青海栽培何首乌中白藜芦醇和白藜芦醇苷提取效果的影响,通过单因素实验和响应面法优化ASE的提取条件,在单因素实验基础上筛选出乙醇浓度、提取温度、提取时间三个主要因素。结果表明,ASE在提取效果上优于其他两种方法,通过响应面分析得出最佳组合条件为:乙醇浓度51%,提取温度96℃、提取时间16 min。快速溶剂萃取法简单、快捷、高效,适用于何首乌中白藜芦醇和白藜芦醇苷的提取。The total content of resveratrol and polydatin was used as the investigation index. The efficiency of ultrasonic extraction,heating reflux extraction and accelerated solvent extraction （ASE） for the extraction of resveratml and polyda- tin in cultivated Polygonum multiflorum from Qinghai was compared. The extraction conditions of ASE were optimized through single-factor test and response surface methodology （RSM）. To obtain the maximum of extraction yield of res- veratrol and polydatin, three main factors including ethanol concentration, extraction temperature and extraction time were selected and further optimized using RSM on the basis of single factor experimental results. As a result, ASE was markedly superior to the other two methods. The optimal conditions of ASE were as follows ：51% of aqueous ethanol, ex- traction temperature of 96 ℃, and extraction time of 16 rain. ASE was simple, rapid and efficient, and was appropriate for the extraction of resveratrol and polydatin in P. multiflorum.

关 键 词：栽培何首乌 白藜芦醇 白藜芦醇苷 快速溶剂萃取 响应面法 

分 类 号：R284.2[医药卫生—中药学]