乙肝病毒衣壳运载体甲胎蛋白启动子驱动的白细胞介素-2在肝癌细胞中的靶向表达  

The targeting expression of IL-2 gene driven by alpha fetoprotein promoter and hepatitis B virus envelope in hepatic carcinoma cell

作　　者：王鹤 邓志华[1] 

机构地区：[1]山西医科大学第二医院消化科,太原030001

出　　处：《肿瘤研究与临床》2014年第1期20-23,共4页Cancer Research and Clinic

摘　　要：目的 利用乙肝病毒衣壳（HBVE）和人甲胎蛋白（AFP）启动子的双重靶向性,观察白细胞介素-2（IL-2）基因是否可以在肝癌细胞中靶向表达.方法 体外培养HepG2、L02、HepG2.2.15细胞,PEG8000浓缩法提取HBVE作为基因运载体,聚合酶链反应（PCR）法制备人AFP启动子-IL-2重组基因,瞬时转染HepG2细胞与L02细胞,反转录-聚合酶链式反应（RT-PCR）观察IL-2 mRNA表达,Western blot观察IL-2蛋白表达情况.结果 以HBVE为基因的运载体,包裹人AFP启动子-IL-2重组基因后转染HepG2细胞和L02细胞,RT-PCR和Westem blot检测到HepG2细胞有IL-2的表达,而对照组L02细胞无IL-2的表达.结论 以HBVE为基因运载体,可以使AFP启动子驱动的IL-2基因在AFP阳性的肝癌细胞靶向表达,从而提高了外源基因导入肝癌细胞的安全性.Objective To observe if IL-2 gene can express in hepatic carcinoma cells by double targeting of hepatitis B virus envelope (HBVE) and alpha fetoprotein (AFP) promoter.Methods HepG2 cells,L02 cells,and HepG2.2.15 cells were cultured in vitro.HBVE was obtained by PEG8000 concentration assay,and the acquired HBVE was used to pack recombintional gene.Human AFP promoter-IL-2 recombinational gene was obtained by PCR.Then HepG2 cells and L02 cells were transfected by transient transfection and the expression of IL-2 was detected by RT-PCR and Western blot.Results IL-2 was detected in HepG2 cells by RT-PCR and Western blot but not in L02 cells.Conclusion By using HBVE as a gene transporter,human AFP promoter-IL-2 recombinational gene can express in hepatic carcinoma cells,thereby it can increase the safety of exogenous gene transfection of hepatic carcinoma cells.

关 键 词：癌 肝细胞 HBVE 甲胎蛋白类 启动区(遗传学) 白细胞介素2 靶向性 

分 类 号：R735.7[医药卫生—肿瘤]