抗虫抗除草剂转基因水稻B1C893的获得与鉴定  

Development and Identification of Herbicide and Insect Resistant Transgenic Plant B1C893 in Rice

作　　者：邓力华[1,2] 邓晓湘[3] 魏岁军 曹正春[3] 唐俐[3] 肖国樱[1] 

机构地区：[1]中国科学院亚热带农业生态研究所亚热带农业生态过程重点实验室,湖南长沙410125 [2]中国科学院大学,北京100049 [3]湖南杂交水稻研究中心,湖南长沙410125

出　　处：《杂交水稻》2014年第1期67-71,75,共6页Hybrid Rice

基　　金：转基因生物新品种培育科技重大专项(2011ZX08001003)

摘　　要：利用农杆菌介导法将Cry1Ca#和Bar基因转化水稻恢复系R893,获得6株再生植株。经过分子鉴定,发现Bar基因和Cry1Ca#基因成功整合到水稻基因组并稳定表达。测定其中单拷贝且可育的转基因株系苗期不同器官中Cry1Ca蛋白的表达量,发现根系中最低而叶片中最高,其中最高的第6号株系叶片中含量达到35.07μg/g。获得的转基因株系对除草剂草铵膦和稻纵卷叶螟均具有良好的抗性。R893, a hybrid rice restorer line, was transformed with the Bar gene and the Cryl Ca# gene by the Agrobacterium-mediated method, and six plantlets were regenerated under glufosinate selection pressure. The mo- lecular analysis of the regenerated plantlets showed that the Bar and Cryl Ca# genes were integrated into the rice ge- nome and stably expressed. The enzyme-linked immunosorbent assay （ELISA） showed that the CrylCa protein ex- pressed in different organs of the fertile transgenic plants with a single copy of exogenous gene at seedling stage. The expression of CrylCa protein was the weakest in root and the strongest in leaf, with the highest value of 35. 073 txg/g in leaf of the transgenic plant B1C893-6. The bioassay exhibited that the transgenic lines were of good resist- ance to both glufosinate and rice leaf roller.

关 键 词：水稻 Cry1Ca#基因 BAR基因 抗除草剂 抗虫 

分 类 号：S511.01[农业科学—作物学] Q78[生物学—分子生物学]