香蕉MuMADS1与泛素激活酶(MuUBA)在采后果实中的相互作用  

The Interaction of Banana MADS-box Protein MuMADS1and Ubiquitin-activating Enzyme E-MuUBA in Post-harvest Banana Fruit

作　　者：张妮[1,2] 刘菊华[2] 贾彩红[2] 张建斌[2] 徐碧玉[2] 金志强[2,3] 

机构地区：[1]海南大学,海南海口570228 [2]中国热带农业科学院热带生物技术研究所农业部热带作物生物技术重点开放实验室,海南海口571101 [3]中国热带农业科学院海口实验站,海南海口570102

出　　处：《热带作物学报》2014年第2期307-312,共6页Chinese Journal of Tropical Crops

基　　金：国家自然科学基金项目(No.30960232);"十二五"农村领域国家科技计划课题(No.2011AA10020605);国家现代香蕉产业技术体系专项资金(No.CARS-32);中央级科研院所基本科研业务费项目(No.ITBB110202)

摘　　要：以香蕉MuMADS1为诱饵载体,采后2 d的香蕉果实的cDNA文库为猎物,采用酵母双杂交的方法得到了泛素激活酶E1的基因片段,命名为MuUBA。采用双分子荧光互补技术进一步验证MuMADS1与MuUBA在植物体内的相互作用。荧光实时定量PCR结果表明,MuMADS1和MuUBA在香蕉中子房发育第4个阶段的表达量最高,但是在茎中的表达量很低,表明其在不同的组织和发育的果实中的表达具有协同性。MuMADS1和MuUBA的表达都受外源乙烯和1-MCP的高度调控,推测MuMADS1和MuUBA在香蕉果实的采后成熟过程中具有很重要的作用。The ubiquitin-activating enzyme E1 gene fragment MuUBA was obtained from banana （Musa acuminata L.AAA） fruit by the yeast two-hybrid method using the banana MADS-box gene MuMADS1 as bait and 2 day post-harvest （DPH） banana fruit cDNA library as prey. Real-time quantitative PCR evaluation of MuMADS1 and MuUBA in banana showed that they were highly expressed in the ovule 4 stage, but presented in low levels in the stem, suggesting a simultaneously differential expression action exists for both MuMADS1 and MuUBA in different tissues and developmental fruits. MuMADS! and MuUBA expression were highly stimulated by exogenous ethylene and suppressed by 1-methylcyclopropene （1-MCP）. These results indicated that MuMADSI and MuUBA were co-regulated in the ethylene signal pathway and might play an important role in post-harvest banana fruit ripening.

关 键 词：香蕉 酵母双杂交 MuMADS1 MuUBA 相互作用 采后果实 

分 类 号：Q789[生物学—分子生物学]