APAAP免疫酶标及DNA末端原位标记双重染色检测急性白血病细胞原位凋亡  

Detection of apoptosis in acute leukemia by double-stained way of APAAP and in situ DNA end labeling

作　　者：李晓[1] 刘薏芝[1] 周艳贞[2] 陶英[1] 石军[1] 杨梅如[1] 浦权[1] 

机构地区：[1]上海市第六人民医院血液科,上海200233 [2]福建省漳州市医院血液科

出　　处：《临床血液学杂志》2001年第1期11-14,共4页Journal of Clinical Hematology

摘　　要：目的 :观察原位细胞凋亡与急性白血病 (AL )发生与转归的关系。方法 :用碱性磷酸酶抗碱性磷酸酶(APAAP)免疫酶标和 DNA末端原位标记 (ISEL )双重染色方法检测 49例急性白血病骨髓涂片中细胞凋亡状况 ,初治组 2 3例与 15例缺铁性贫血病例对照并与 2 6例经治病例对照。结果 :未经治疗的 AL 平均凋亡指数 (AI)明显低于对照组 (P <0 .0 1)。化疗后凋亡明显增加 ,与化疗前比较差异有极显著性意义 (P <0 .0 1)。AI>5 %者平均原始细胞下降指数 (MBDI)为 2 8.6 8% ,AI<5 %者平均 MBDI仅为 4.9%。凋亡细胞大多数为白血病细胞。非白血病细胞凋亡亦有增加。结论 :AL 发生与凋亡逃逸有关 ,细胞毒化疗的主要机理之一为促进细胞凋亡。化疗后全血细胞下降与正常造血细胞凋亡增加有关。Objective:To explore the relation ship between apoptosis and pathogenesis and transition of acute leukemia (AL).Methods:Aridouble stained way of APAAP and in situ DNA end labeling (ISEL) was used to detect apoptosis in 49 cases of acute leukemia,and in 15 cases of iron deficiency anemia(IDA) as control.Results:The mean apoptosis index(AI) in untreated AL cases was much lower than that in control group(P< 0.01 ). The undertreated cases had a greatly increased mean AI(P< 0.001 ) which showed a positive correlation with marrow blast decrease index(MBDI) . Apoptosis occurred mainly in leukemia cells, but a simultaneous growth apoptosis of non leukemia cells was also observed. Conclusions:The pathogenesis of acute leukemia relates to apoptosis escape. One of the important mechanism of cellulotoxic chemotherapy is to promote apoptosis of leukemia cell and chemotherapy induced pancytopenia is caused partly by increased apoptosis of non leukemia cells.

关 键 词：免疫酶技术 急性白血病 ISEL 双重染色检测 

分 类 号：R733.71[医药卫生—肿瘤]