Digestion performance and microbial community in full-scale methane fermentation of stillage from sweet potato-shochu production  

作　　者：Tsutomu Kobayashi Yueqin Tang Toyoshi Urakami Shigeru Morimura Kenji Kida 

机构地区：[1]Graduate School of Science and Technology, Kumamoto University, 2-39-1 Kurokami, Chuo-ku, Kumamoto 860-0862, Japan [2]Kirishima Shuzo Co., Ltd 4-28-1 Shimokawahigashi, Miyakonojo 885-8588, Japan [3]College of Architecture and Environment, Sichuan University

出　　处：《Journal of Environmental Sciences》2014年第2期423-431,共9页环境科学学报（英文版）

摘　　要：Sweet potato shochu is a traditional Japanese spirit produced mainly in the South Kyushu area in Japan. The amount of stillage reaches approximately 8 x 105 tons per year. Wastewater mainly containing stillage from the production of sweet potato-shochu was treated thermophilically in a fullscale treatment plant using fixed-bed reactors （8 reactors ×283 m3）. Following the addition of Ni2＋ and Co2＋, the reactors have been stably operated for six years at a high chemical oxygen demand （COD） loading rate of 14 kg/（m3.day）. Analysis of coenzyme content and microbial communities indicated that similar microbial communities were present in the liquid phase and on the fiber carders installed in reactors. Bacteria in the phyla Firmieutes as well as Bacteroidetes were dominant bacteria, and Methanosarcina thermophila as well as Methanothermobacter crinale were dominant methanogens in the reactors. This study reveals that stillage from sweet potato-shochu production can be treated effectively in a full-scale fixed-bed reactor under thermophilic conditions with the help of Ni2~ and Co2＋. The high diversity of bacterial community and the coexistence of both aceticlastic and hydrogenotrophic methanogens contributed to the excellent fermentation performance.Sweet potato shochu is a traditional Japanese spirit produced mainly in the South Kyushu area in Japan. The amount of stillage reaches approximately 8 x 105 tons per year. Wastewater mainly containing stillage from the production of sweet potato-shochu was treated thermophilically in a fullscale treatment plant using fixed-bed reactors （8 reactors ×283 m3）. Following the addition of Ni2＋ and Co2＋, the reactors have been stably operated for six years at a high chemical oxygen demand （COD） loading rate of 14 kg/（m3.day）. Analysis of coenzyme content and microbial communities indicated that similar microbial communities were present in the liquid phase and on the fiber carders installed in reactors. Bacteria in the phyla Firmieutes as well as Bacteroidetes were dominant bacteria, and Methanosarcina thermophila as well as Methanothermobacter crinale were dominant methanogens in the reactors. This study reveals that stillage from sweet potato-shochu production can be treated effectively in a full-scale fixed-bed reactor under thermophilic conditions with the help of Ni2~ and Co2＋. The high diversity of bacterial community and the coexistence of both aceticlastic and hydrogenotrophic methanogens contributed to the excellent fermentation performance.

关 键 词：thermophilic methane fermentation stillage treatment full-scale fixed-bed reactor microbial community 

分 类 号：TS262[轻工技术与工程—发酵工程]