核因子-κB在BN大鼠脉络膜新生血管中的表达  

Expression of NF-κB in choroidal neovascularization of BN rat

作　　者：高小燕[1] 何守志[2] 

机构地区：[1]北方工业大学医院眼科,北京市100144 [2]解放军总医院眼科,全军眼科中心,北京市100853

出　　处：《眼科新进展》2014年第2期114-118,共5页Recent Advances in Ophthalmology

基　　金：全军"十五"科研基金资助项目(编号:02M014)~~

摘　　要：目的观察核因子-κB(nuclear factor-kappa B,NF-κB)在氪激光诱导的BN大鼠脉络膜新生血管(choroidal neovascularization,CNV)中的表达。方法健康雄性BN大鼠48只,随机选择一眼作为实验眼,建立CNV动物模型。于光凝后1周、2周、3周、4周、5氉周、6周、7周、8周行FFA检查后,分别于不同时间点各摘除6只BN大鼠的眼球,检测NF-κB蛋白和单核细胞趋化蛋白-1(monocyte chemoattractant protein-1,MCP-1)的表达及VEGF mRNA的情况,并测量不同时期CNV面积及FFA检查时荧光渗漏情况。结果病理切片观察及FFA检测发现,光凝后CNV逐渐形成,CNV面积随时间推移不断增大,不同时间点间CNV面积差异均有统计学意义(均为P<0.05)。免疫组织化学检测结果表明,光凝后NF-κB蛋白、MCP-1蛋白的表达均显著增加,不同时间点间差异均有统计学意义(均为P<0.05)。原位杂交检测结果表明,光凝后不同时间VEGF mRNA的表达逐渐增强,各时间点间差异均有统计学意义(均为P<0.05)。相关性研究结果表明,NF-κB蛋白表达情况与CNV面积、MCP-1、VEGF mRNA及FFA(IOD)均呈显著正相关(r=0.951、0.924、0.976、0.824,均为P<0.05)。结论 NF-κB可能在CNV发生发展中起了很重要的作用,可能成为将来治疗CNV很好的靶点。Objective To observe the expression of nuclear factor-kappa B （ NF-κB） in choroidal （CNV） induced by krypton of Brown Norway（BN） rat. Methods One eye of 48 Healthy male BN rats was chosen randomly as experi- mental eye,the other eye as normal eye. CNV models were established, FFA examina- tion was made at postoperative 1 week,2 weeks,3 weeks,4 weeks,5 weeks,6 weeks,7 weeks ,8 weeks,then eyes of 6 BN rats were enucleated at each time point for histologi- cal slices. The expression of NF-κB, monocyte chemoattractant protein-1 （ MCP-1 ） were examined by immunohistochemistry, the expression of vascular endothelial growth fac- tor （VEGF）mRNA was observed by in situ hybridization, the most area of CNV was measured and integrated optical density（IOD） of fluorescence leakage was observed by FFA.Results CNV gradually formed after photocoagulation, the area of CNV in- creased with the time prolong, there were statistical differences in CNV area between different time points （all P 〈 0.05 ）. The expression of NF-κB and MCP-1 protein all in- creased after photocoagulation, there were statistical differences in CNV area between different time points （ all P 〈0.05 ）. The expression of VEGF mRNA gradually strength- ened,there were statistical differences in CNV area between different time points （ all P 〈 0.05 ）. There were positive correlation between NF-κB protein expression and CNV area,MCP-1 protein,VEGF mRNA,FFA （IOD） （ r = 0. 951,0. 924,0. 976,0. 824, all P 〈 0. 05 ）. Conclusion NF-κB plays an important role in the development of CNV, which may be one of the prospective targets in treating CNV.

关 键 词：脉络膜新生血管 核因子-ΚB 单核细胞趋化蛋白-1 血管内皮生长因子 大鼠 

分 类 号：R774.5[医药卫生—眼科]