大蒜油联合5-FU诱导腺样囊性癌细胞株ACC-M凋亡的研究  

Study on garlic oil combined with 5-FU induced apoptosis of adenoid cystic carcinoma cell line ACC-M

作　　者：吴发印[1] 周鹤峰[2] 樊治英[1] 朱雅文[1] 李永烨[3] 姚裕坤 冉丹[3] 

机构地区：[1]遵义医学院第五附属珠海医院口腔科广东珠海,519100 [2]遵义医学院珠海校区生物系 [3]遵义医学院珠海校区口腔系

出　　处：《临床耳鼻咽喉头颈外科杂志》2014年第3期175-177,181,共4页Journal of Clinical Otorhinolaryngology Head And Neck Surgery

基　　金：贵州省科学技术基金项目[No:黔科合J字LKZ(2010)07号];遵义医学院大学生创新性实验计划项目(No:D-12-11)

摘　　要：目的:探讨大蒜油联合5-FU诱导腺样囊性癌细胞株ACC-M凋亡的效应。方法:人涎腺腺样囊性癌细胞株ACC-M细胞培养,分为实验组(5-FU组、大蒜油组、大蒜油+5-FU组)和对照组,采用MTT法观察肿瘤细胞的体外生长活性;采用流式细胞术分析肿瘤细胞周期和凋亡率的变化。结果:MTT实验结果显示不同浓度的5-FU、大蒜油、大蒜油+5-FU对ACC-M细胞均有抑制作用,随着浓度和作用时间的增加而呈上升趋势;流式细胞术分析显示细胞周期发生明显变化,随着浓度增加、作用时间延长G0/G1期的细胞比率上升,S期无明显变化,而G2/M期的细胞下降,凋亡率显示大蒜油联合5-FU诱导ACC-M细胞凋亡明显强于单独组。结论:大蒜油能有效诱导腺样囊性癌细胞株ACC-M凋亡,大蒜油联合5-FU应用对ACC-M细胞的作用明显强于大蒜油,5-FU可单独应用。Objective： To Observe the effect of garlic oil combined with 5 FU induced apoptosis of adenoid cystic carcinoma cell line ACC-M. Method： Human salivary adenoid cystic carcinoma cell line ACC M was cultured, divided into the experimental group（5-FU group, garlic oil group, garlic oil＋5-FU group） and the control group, to observe the growth activity of tumor cells by MTT methods;to analyse the changes of cell cycle and apoptosis rate by flow cytometry. Result： MTT experiments showed that 5-FU, garlic oil, garlic oil and ＋ 5-FU on ACC-M cells have inhibition in different concentration, with the increase of concentration and action time of the rise; Cell cycle analysis showed significant changes in flow cytometry. With the increase of concentration and the acting time, the G0/G1 phase of the cell ratio increased, S had no significant change, but G2/M phase cells decreased. Apoptosis rate display showed garlic oil combined with 5-FU induced apoptosis of ACC-M cells was significantly stronger than single group. Conclusion： Garlic oil can effectively induce the apoptosis of adenoid cystic carcinoma FU on ACC-M cells was stronger than the garlic oil, 5

关 键 词：大蒜油 5-FU 癌 腺样囊性 凋亡 

分 类 号：R730.261[医药卫生—肿瘤]