多微孔多聚糖止血粉应用于软组织创伤性出血  

Hemostasis effects of microporous polysaccharide hemostatic powder on soft tissue trauma

作　　者：史跃[1] 杜宝堂 何远清[3] 殷文静[3] 

机构地区：[1]解放军第97医院介入放射科,江苏省徐州市221004 [2]江苏奥科生物科技有限公司,江苏省镇江市212003 [3]江苏大学实验动物中心,江苏省镇江市212002

出　　处：《中国组织工程研究》2014年第3期406-411,共6页Chinese Journal of Tissue Engineering Research

基　　金：镇江市政府331工程资助~~

摘　　要：背景:前期实验以马铃薯为原料经交叉乳化制备了一种具有自主知识产权的专利产品多微孔多聚糖止血材料。目的:观察多微孔多聚糖止血粉应用于软组织创伤出血的止血效果。方法:在实验组1家兔腹部软组织制作1条长约3 cm、深约0.5 cm的创面,吸干出血创面后将多微孔多聚糖止血粉直接喷洒于创面,用量1.0-2.0 g,以吸干出血创面后不做任何处理的家兔为对照。在实验组2家兔腹部组织切割1条长约3 cm、深约1.0 cm的创面,吸干出血创面后将多微孔多聚糖止血粉均匀喷洒于创面,用量1.0-2.0 g,以吸干出血创面后不做任何处理的家兔为对照。结果与结论:多微孔多聚糖止血粉吸收血液中的水分,形成"糊状凝胶"止血痂黏附于出血创面表面达到止血效果。实验组1创面止血时间(15.25±1.04)s,实验组2创面止血时间(11.25±1.89)s,止血显效率为87.5%,有效率为100%;对照组创面止血时间大于5 min。喷洒多微孔多聚糖止血粉24 h苏木精-伊红染色显示,肌组织轻度水肿,小血管扩张,间质内可见少数散在中性粒细胞浸润;7 d时炎症消退,肌组织表面轻度纤维化、完全吸收,接近正常组织,肌细胞未见明显变化。以上结果表明多微孔多聚糖止血粉可用于软组织创伤止血。BACKGROUND： A cross-emulsification patented product of microporous polysaccharide hemostatic powder was prepared using potatoes as raw material with independent intellectual property rights. OBJECTIVE： To observe hemostasis effects of microporous polysaccharide hemostatic powder on soft tissue trauma. METHODS： A wound, about 3 cm long and 0.5 cm deep, was made on the abdominal soft tissue of rabbits in the experimental group 1, and then microporous polysaccharide hemostatic powder, 1.0-2.0 g, was sprayed directly on the wound. In the experimental group 2, a wound, about 3.0 cm long and 1.0 cm deep, was made on the abdominal soft tissue of rabbits, and then microporous polysaccharide hemostatic powder, 1.0-2.0 g, was sprayed directly on the wound. Another rabbits with untreated wound served as controls. RESULTS AND CONCLUSION： Microporous polysaccharide hemostatic powder formed a ＂pasty gel＂ covering the bleeding wound that achieved hemostatic effect. The bleeding time was （15.25±1.04） seconds in the experimental group 1 and （11.25±1.89） seconds in the experimental group 2. The marked effective and effective rates for hemostasis were 87.5% and 100%, respectively. In the control group, the bleeding time was more than 5 minutes. Hematoxylin-eosin staining showed mild muscle edema, vasodilatation of small blood vessels, and few scattered endoplasmic neutrophils infiltrated at 24 hours after treatment with microporous polysaccharide hemostatic powder; till the 7th day, inflammation subsided, mild fibrosis was visible on muscle tissue surface, the hemostatic powder was completely absorbed, the wound tissue was close to the normal tissue, and there were no significant changes in muscle cells. These findings suggest that microporous polysaccharide hemostatic powder can be used for hemostasis of soft tissue trauma.

关 键 词：生物材料 材料相容性 马铃薯淀粉 止血材料 多微孔多聚糖 实验 家兔 

分 类 号：R318[医药卫生—生物医学工程]