海藻酸钠-壳聚糖微胶囊载体在组织工程研究中的应用  

Alginate-chitosan microcapsule in tissue engineering research

作　　者：姜恒丽[1,2] 崔元璐[1,2] 齐学洁[1,2] 齐云[3] 丁舒[3] 

机构地区：[1]天津市现代中药重点实验室(国家重点实验室培育基地),天津市300193 [2]天津中医药大学中医药研究院,天津市300193 [3]天津大学环境科学与工程学院,天津市300193

出　　处：《中国组织工程研究》2014年第3期412-419,共8页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金项目(51108310);教育部高等学校博士学科点专项科研基金项目(20091210110003);天津市科技兴海计划项目(KX2010-0005)~~

摘　　要：背景:用壳聚糖包裹海藻酸钠制备微囊,可以改善海藻酸钠水凝胶的力学性能,如何获得理想的海藻酸钠壳聚糖微囊以及该微囊体系的应用前景是这一研究的关键。目的:探讨海藻酸钠壳聚糖微胶囊载体的制备方法、成型机制,分析影响微胶囊膜强度性能的几个重要因素及探讨海藻酸钠-壳聚糖微胶囊在固定化细胞技术、药物载体和组织工程方面的应用前景。方法:由第一作者采用计算机检索PubMed数据库、Elsevier ScienceDirect、中国知网库、万方数据库1987至2013年有关海藻酸钠壳聚糖微囊制备方法、反应机制及应用前景的文章。结果与结论:海藻酸盐水凝胶在药物释放和组织工程领域具有很多优势,但是凝胶溶蚀现象和力学性能缺陷限制了它的应用,壳聚糖与海藻酸钠通过静电相互作用形成聚电解质络合物,弥补了海藻酸钠凝胶的不足。通过控制壳聚糖溶液的性质-壳聚糖的分子质量、壳聚糖溶液的pH值和浓度制备膜强度高的微囊,海藻酸钠-壳聚糖微囊在固定化技术、药物释放和组织工程领域表现出了广阔的应用前景。BACKGROUND： Alginate-chitosan microcapsule can improve the mechanical property of sodium alginate hydrogels. How to obtain the ideal sodium alginate-chitosan microcapsule and the prospect for application of the microcapsule system is the key to this study. OBJECTIVE： To investigate the preparation method and formation mechanism of alginate-chitosan microcapsules, to analyze several important factors affecting the strength of the microcapsule membrane, and to explore the prospects of alginate-chitosan microcapsules in immobilized cell technology, in tissue engineering and as a drug carrier. METHODS： The first author searched PubMed, Elsevier ScienceDirect, CNKI and Wanfang database （1987/2013） to retrieve literatures about the preparation method, formation mechanism and application prospect of alginate-chitosan microcapsules. RESULTS AND CONCLUSION： Sodium alginate hydrogels have many advantages in drug release and tissue engineering, but its application is limited by gel dissolution phenomena and deficiencies in its mechanical properties. Chitosan-alginate microcapsules make up for the deficiency of sodium alginate hydrogels by electrostatic interactions to form polyelectrolyte complexes. By controlling the nature of the chitosan solution--the molecular weight of chitosan, pH and concentration of chitosan solution, we can prepare the microcapsules with high film strength. Alginate-chitosan microcapsules have shown broad application prospects in immobilization technology, drug release and tissue engineering.

关 键 词：生物材料 缓释材料 海藻酸钠 壳聚糖 微胶囊 固定化技术 药物释放 组织工程 国家自然科学基金 

分 类 号：R318[医药卫生—生物医学工程]