系统性红斑狼疮小鼠骨髓间充质干细胞的成骨、成脂分化能力下降  

Bone marrow mesenchymal stem cells from systemic lupus erythematosus mice have reduced osteogenic and adipogenic abilities

作　　者：阮光萍[1] 王金祥[1] 杨建勇[1] 刘菊芬[1] 蔡学敏[1] 庞荣清[1] 吕燕波[1] 潘兴华[1] 

机构地区：[1]解放军成都军区昆明总医院干细胞工程实验室,云南省昆明市650032

出　　处：《中国组织工程研究》2014年第1期1-6,共6页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金资助项目(31172170);中国博士后科学基金特别资助项目(201104748);云南省高新技术产业发展专项(201204)~~

摘　　要：背景:系统性红斑狼疮患者的骨髓间充质干细胞与正常人相比是否有不同,相关报道较少。目的:对比系统性红斑狼疮模型小鼠与正常小鼠的骨髓间充质干细胞多向分化能力的差别。方法:分离培养系统性红斑狼疮模型小鼠与C57BL小鼠的骨髓间充质干细胞(对照组),分别进行成骨、成脂诱导分化,观察两种小鼠的骨髓间充质干细胞的分化能力。结果与结论:C57BL小鼠的骨髓间充质干细胞经传代后,为长梭形,呈均匀分布生长;系统性红斑狼疮模型小鼠的骨髓间充质干细胞表现为生长缓慢,细胞相对C57BL小鼠要少一些。经过成骨诱导显示系统性红斑狼疮模型小鼠的钙结节和钙盐明显少于对照组,PCR检测成骨基因Runx2,碱性磷酸酶,骨钙素表达也明显下降。经过成脂诱导显示系统性红斑狼疮模型小鼠的脂滴明显少于对照组,PCR检测成脂基因PPARγ2,脂蛋白酯酶表达也明显下降。说明系统性红斑狼疮模型小鼠的骨髓间充质干细胞成骨与成脂能力都低于C57BL小鼠,系统性红斑狼疮模型小鼠的骨髓间充质干细胞的多向分化能力受损。BACKGROUND：There are less studies addressing whether bone marrow mesenchymal stem cells from systemic lupus erythematosus patients are different from healthy people. OBJECTIVE：To compare the multi-differentiation capacity of bone marrow mesenchymal stem cells isolated from systemic lupus erythematosus model mice and normal control mice. METHODS：Bone marrow mesenchymal stem cells of systemic lupus erythematosus model mice and C57BL mice were isolated and cultured fol owed by osteogenic and adipogenic differentiations, respectively. Differentiation abilities of two kinds of mouse bone marrow mesenchymal stem cells were observed. RESULTS AND CONCLUSION：Passaged bone marrow mesenchymal stem cells from C57BL mice were long spindle-shaped and evenly distributed, while bone marrow mesenchymal stem cells from systemic lupus erythematosus model mice showed slow growth and were relatively smal er than those from C57BL mice. After osteogenic induction, the amount of calcium salt and calcium nodules were significantly less in the bone marrow mesenchymal stem cells from systemic lupus erythematosus model mice than from normal control mice. PCR detection showed that expressions of Runx2, alkaline phosphatase and osteocalcin were also significantly decreased in the bone marrow mesenchymal stem cells from systemic lupus erythematosus model mice. After adipogenic induction, the number of lipid droplets in the bone marrow mesenchymal stem cells from systemic＆amp;nbsp;lupus erythematosus model mice was significantly less than the control group, and PCR detection also showed significantly decreased expression of adipogenic genes, including PPARγ2 and lipoprotein lipase. These findings suggest that the bone marrow mesenchymal stem cells from systemic lupus erythematosus model mice exhibit lower osteogenic and adipogenic capacities than those from normal C57BL mice, and also have the impaired multi-differentiation ability.

关 键 词：干细胞 骨髓干细胞 骨髓间充质干细胞 系统性红斑狼疮 成骨分化 成脂分化 RUNX2 碱性磷酸酶 骨钙素 PPARΓ2 脂蛋白酯酶 国家自然科学基金 

分 类 号：R758.62[医药卫生—皮肤病学与性病学]