激光散斑成像监测C57小鼠皮质血流及光化学缺血变化  

Laser Speckle Imaging of Mice Cerebral Blood Flow and Cortical Infarction

作　　者：方瑜[1] 邱占东[1] 闵哲[1] 熊永洁[1] 胡文涛[1] 黄梁江[1] 张苏明[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院神经内科,武汉430030

出　　处：《神经损伤与功能重建》2014年第1期16-19,共4页Neural Injury and Functional Reconstruction

基　　金：武汉市科技计划项目(No.2013060501010159)

摘　　要：目的：探索激光散斑成像（LSI）技术在监测C57BL／6小鼠脑皮质血流中的应用，初步观察光化学脑梗死模型的脑皮质血流改变。方法：采用8只8～10周雄性C57BL／6小鼠，麻醉后切开头皮置于LSI系统下观察脑皮质血流变化。5只8～10周雄性C57BL／6小鼠制作光化学梗死灶模型，分别在光化学造模前及造模后24h观察脑皮质血流变化，同时5只8～10周雄性C57BL／6小鼠假手术作为假手术组。结果：8只C57小鼠可以在不损伤颅骨的条件下实现较长时间的脑血流成像检查，具有较高成像质量，可以辨别脑皮质小血管血流变化。5只小鼠光化学造模取得成功，利用LSI可以观察到24h后脑皮质梗死区血流量变化，部分小血管血流减少或消失。尼氏染色显示手术组小鼠皮质脑梗死形成。结论：LSI可以观察C57小鼠脑皮质血流变化，具有较高的空间及时间分辨率。Objective：To explore the application of laser speckle imaging in monitoring C57BL/6 mice cerebral blood flow under normal condition and the photochemical infarction. Methods： Eight 8-10 weeks male C57BL/6 mice were used for blood flow detection. The cortex blood flow of the C57BL/6 mice was monitored by laser speckle imaging system for 30 minutes. Five 8-10 weeks male C57BL/6 mice were induced cortical photochemi- cal infarct while five 8-10 weeks male C57BL/6 mice in sham group received sham operation. Laser speckle imaging was preformed before and 24 hs after the surgery. Results： Eight C57 mice can be detected cerebral blood flow imaging with high image quality without damaging the skull. The small blood vessels can be observed by this system. In the photothrombosis group, small blood flow was decreased or disappeared on the cortex of mice using laser speckle image. Conclusion： The image of C57 mice brain cortical blood flow could be obtained by laser speckle imaging with high spatial and temporal resolution.

关 键 词：激光散斑成像 脑梗死 脑血流 

分 类 号：R741[医药卫生—神经病学与精神病学] R741.04[医药卫生—临床医学]