载顺铂聚乳酸静电纺丝膜对口腔鳞癌细胞的杀伤作用研究  

Polylactic acid containing cisplatin electrostatic spinning membrane for cytotoxicity study of oral squamous cell carcinoma cell in vitro

作　　者：周丽佳[1] 杨洲[2] 张士博[1] 郝秀峰[2] 张泽兵[1] 

机构地区：[1]吉林大学口腔医院,吉林长春130021 [2]吉林大学化学院,吉林长春130012

出　　处：《中国实验诊断学》2014年第2期179-182,共4页Chinese Journal of Laboratory Diagnosis

基　　金：吉林省科技发展计划项目(201105101)

摘　　要：目的探讨载顺铂聚乳酸静电纺丝膜对口腔鳞癌CAL-27细胞的杀伤作用。方法利用静电纺丝技术将顺铂(DDP)、聚乳酸(PLA)混合溶液制成载顺铂聚乳酸静电纺丝膜(DDP/PLA膜)。通过扫描电镜(SEM)观察DDP/PLA膜表观形态,紫外分光光度分析法测定PLA膜的降解速率,MTT法观察细胞的增殖活性。实验分为空白对照组,DDP组,PLA膜组,DDP/PLA膜组。结果 SEM可以观察到纳米纤维表面光滑、直径均匀;降解速率显示聚乳酸静电纺丝膜在第一天降解50%左右,以后降解缓慢,在第13天降解趋于完毕;细胞增殖活性结果显示:不含药物的纯膜对细胞基本无影响,不具有统计学意义(P>0.05);DDP组、DDP/PLA组抑制细胞增殖活性,有统计学差异(P<0.05);实验前3天DDP组作用效果好于DDP/PLA膜组,3天后DDP/PLA膜组作用效果优于DDP组,与DDP组相比,相同剂量的DDP/PLA组对细胞的杀伤作用时间延长。结论 DDP/PLA膜可以杀伤口腔鳞癌CAL-27细胞,3天后的作用效果优于DDP组;DDP/PLA膜具有缓释作用,作用时间较DDP组长。Objective To investigate cisplatin containing polylactic acid electrospun membranes for cytotoxicity study oral cancer CAL-27 cells in vitro. Methods Making cisplatin （DDP） and PLA mix together to fabricate polylactic acid containing cisplatin nanofibers （DDP/PLA） membrane by electrospinning method. The micro morphology was ob- served by scanning electron microscope（SEM）,the degradation rate of PLA membrane by UV spectrophotometric, the proliferation activity of CAL-27 cells was observed by tetrazolium bromide （MTT） colorimetry,The CAL-27 cells were divided into conntrol group,DDP group,PLA membrane group,DDP/PLA membrane group. Results Nanofibers was smooth and uniform diameter through SEM; On the first day, the degradation rate of PLA membrane was about 50 %, after the degradation rate was slow and degradation tends to completely In the thirteenth day. The MTT result ＇showed that the cell proliferation in PLA membrane group was no significant impact（P〈0. 05）, DDP group and DDP/PLA group could inhibit cell proliferation,which was statistic significance（P〈0.05）. Three days of beginning of the experi- ment, the effect of cell killing treat with DDP was better than DDP/PLA group, three days later, the effect of cell killing treat with DDP membrane was better than DDP group. Campared with the DDP group, the same dose of DDP/PLA group on cell killing effect was longer. Comclusion DDP/PLA membrane could kill cells in oral squamous cell carcino- ma CAL-27,three days later, the effect of cell killing treat with DDP membrane was better than DDP group;DDP/PLA nanofibers membrane owns good controlled release effect and has better effect than DDP group.

关 键 词：静电纺丝 聚乳酸 顺铂 CAL-27细胞 

分 类 号：R739.8[医药卫生—肿瘤]