类人胶原蛋白-微孔淀粉的制备及生物学评价  

The preparation and biological evaluation of human-like collagen-microporous

作　　者：蒙俊桦 王琪凯[1] 范代娣[1] 段志广[1] 乔存存[1] 王溢萍 

机构地区：[1]西北大学化工学院,陕西西安710069

出　　处：《西北大学学报（自然科学版）》2014年第1期61-65,共5页Journal of Northwest University（Natural Science Edition）

基　　金：国家大学生创新性实验计划基金资助项目(2012-1069-7015)

摘　　要：文中以微孔淀粉为主体,研究一种新型复合止血材料的制备及生物学评价。此复合材料由一定比例的α-淀粉酶与糖化酶复合后处理淀粉以形成微孔结构,然后复合类人胶原蛋白,再以戊二醛为交联剂进行交联;用SD大鼠作肝脏出血模型进行止血效果对比试验。通过正交试验确定微孔淀粉最优制备工艺条件如下:α-淀粉酶:糖化酶=1∶3,T/℃=50,t/h=20,pH=4.8;用0.2%(V/V)戊二醛乙醇溶液交联的类人胶原蛋白-微孔淀粉止血材料,在相同的止血时间下,该复合材料平均吸水率为3 580%,对照组(分别是微孔淀粉和类人胶原蛋白海绵)的平均吸水率为3 160%和3 280%。而在SD大鼠肝脏实验中,复合材料t/s=94±13,而外购的止血剂t/s=121±15。类人胶原蛋白-微孔淀粉复合材料止血的止血原理从3个方面展开:①微孔淀粉吸收水分并将血液中的有形物质聚集到表面;②加速内源性凝血因子的激活使其形成血栓;③在类人胶原蛋白的牵引下,淀粉颗粒聚成团大幅度增加了比表面积,使止血效果更优秀;对照组止血机理只包含了该复合材料的前两个方面。结果显示,复合材料的止血效果要优于普通微孔淀粉制成的止血剂,证明该复合止血材料具有良好的止血性能。Focusing on the microporous starch, the research on a new composite material preparation and the biological evaluation of hemostasis have been done. The composite material is made of certain proportion of α- am-ylase and glucoamylase, prepared by mixing microporous starch, which use the glutaraldehyde method hu- man-like collagen （HLC） crosslinking, through glutaraldehyde crosslinking, then use SD rats with liver hemor- rhage model for comparative tests detect bleeding. Consequently, the results show that the optimum conditions is α-amylase ： Glucoamylase = 1 ： 3, Tempreture/℃ = 50, Time/h = 20, pH = 4.8. Then Adding 0.2% （ V/ V） ethanol solution of glutaraldehyde to composite microporous starch and HLC. At the same time of hemosta- sis, the composite＇s average water absorption is 3 580% , meanwhile, the control group （which are micro- porous starch and HLC sponge） are 3 160% and 3 280%. In rat liver hemostasis experiments show that the composited material of Time/s = 94 ＋ 13, however, the microporous starch purchased of Time/s = 121 ＋ 15. As the composited material, the principle of hemostasis promote to three factors to undertake as follow ： （1）mi- croporous starch absorbs water from the blood, furthermore, gather tangible substance on the surface ; （2）accel- erating the activation of clotting factor endogenous take form of thrombus;（3）In the interaction of HLC, the granules of microporous starch, dramatically, increase specific surface area, showed the composite is pretty material for the area hemostatic. By the contrast, the control group has the first two aspects of the composited material only. From results, the material is better than the control group. The results show that the composited material has good effect on hemostatic area.

关 键 词：微孔淀粉 类人胶原蛋白 止血材料 生物相容性 

分 类 号：R318.08[医药卫生—生物医学工程]