高抗逆高丁比拜氏梭菌的选育及其性能考察  

Screening of Clostridium beijerinckii mutant with high inhibitor tolerance and high proportions of butanol for butanol production

作　　者：贺爱永[1] 尹春燕[1] 孔祥平[1] 陈佳楠[1] 姜岷[1] 吴昊[1] 

机构地区：[1]南京工业大学生物与制药工程学院 材料化学工程国家重点实验室,南京210009

出　　处：《生物加工过程》2014年第1期23-27,共5页Chinese Journal of Bioprocess Engineering

基　　金：国家重点基础研究发展计划(973计划)(2011CB707405);材料化学工程国家重点实验室基金;江苏省"青蓝工程";林业公益性专项(201004001);国家自然科学基金青年基金(21106067);江苏省普通高校研究生科研创新计划(CXZZ13_04)

摘　　要：以抗逆突变株Clostridium beijerinckii IB4为出发菌株,通过常压室温等离子体诱变(ARTP),刃天青平板初筛,摇瓶发酵复筛,筛选出1株高抗逆高丁比的突变菌株C.beijerinckii IT111。发酵结果表明:该突变菌株利用多种C源时均展现其高丁醇比的特性,以玉米芯酸解糖液为C源时,溶剂产量达到10.5 g/L,丁醇8.0 g/L,丁醇比高达76%。抑制物抗逆性测试结果显示:糠醛和酸类对C.beijerinckii发酵影响较小,酚类物质对C.beijerinckii抑制作用较强,其中以香草醛为最。综上所述,C.beijerinckii IT111是1株极具潜力的利用木质纤维原料制备丁醇的菌株。Clostridium beijerinckii IT111,a tolerance mutant strain with high proportions of butanol,was obtained by atmospheric and room temperature plasma （ ARTP ） and high-throughput screening.Fermentation results showed that the mutant had a high butanol proportions using a variety of carbon sources.When non-detoxified hemicellulosic hydrolysate of corn fiber treated with dilute sulfuric acid （ SAHHC） was used as a substrate,total solvent（ ABE） and butanol production of 10.5 g/L and 8.0 g/L were obtained,respectively.The butanol proportion was up to 76%.The results of toxicity evaluation of model inhibitors showed that acetates,furfural and 5-HMF were not inhibitory to ABE production,while phenolic compounds were potent inhibitors of growth and ABE production, especially vanillin.C.beijerinckii IT111 was a promising strain for ABE production from lignocellulosic materials.

关 键 词：拜氏梭菌 丁醇 等离子体诱变 木质纤维素 

分 类 号：TQ921[轻工技术与工程—发酵工程]