木质纤维素酶基因资源挖掘及真菌酶系改造  

Mining resource of lignocellulase genes and optimizing fungal enzyme system

作　　者：邹根[1] 刘睿[1] 魏勇军[1] 周志华[1] 严兴[1] 

机构地区：[1]中国科学院 上海生命科学研究院 植物生理生态研究所 合成生物学重点实验室,上海200032

出　　处：《生物加工过程》2014年第1期63-71,共9页Chinese Journal of Bioprocess Engineering

基　　金：国家重点基础研究发展计划(973计划)(2011CB707400)

摘　　要：简述了木质纤维素酶基因资源挖掘的策略和方法及其在丝状真菌酶系改造中的应用。从候选基因的获取(木质纤维素酶基因资源的挖掘和高效利用)、外源基因的表达、酶系的复配和重构等方面综述了丝状真菌酶系改造的最新进展,并提出了丝状真菌酶系改造中亟须解决的关键问题。The strategy and the method for mining resource of lignocellulase genes,and its application in optimization of enzyme system of filamentous fungi were reviewed.Recent progress in mining and utilization resource of lignocellulase genes,heterologous expression of lignocellulosic genes,reconstruction of enzyme system and other issues about optimizing fungal enzyme system were introduced.Key issues in optimizing fungal enzyme system were discussed.

关 键 词：木质纤维素 酶系 丝状真菌 

分 类 号：Q938.1[生物学—微生物学]