Busuishengxue ranules mediate their effects upon non-severe aplastic anemia via mitogen-activated protein kinase/extracellular signal-regulated kinase pathway  

作　　者：Jinhuan Wang Feng Sun Weizheng Sun Yanli Yong Haitao Shi Sijia Liu Limei Liu 

机构地区：[1]Department of Hematology,First Affiliated Hospital,Heilongjiang University of Chinese Medicine [2]Department of Traditional Chinese Medicine,Longnan Hospital of Daqing City [3]Department of Encephalopathy,Chinese Medicine Hospital of Harbin [4]Renal Department of Internal Medicine,Heilongjiang Provincial Academy of Traditional Chinese Medicine

出　　处：《Journal of Traditional Chinese Medicine》2014年第1期23-29,共7页中医杂志（英文版）

基　　金：Supported by the National Natural Science Foundation of China(No.81202680);Specialized Research Fund for the Doctoral Program of Higher Education(No.200802280003,20092327120001);China Postdoctoral Science Foundation(20100481034);Heilongjiang administration of Traditional Chinese Medicine Foundation(ZHYO-W42);Heilongjiang University of Chinese Medicine Foundation(No.200901)

摘　　要：OBJECTIVE:To observe the clinical efficacy of Busuishengxue granules on non-severe aplastic anemia(NSAA)and investigate its effect on the mitogen-activated protein kinase/extracellular signal-regulated kinase(MAPK/ERK)pathway.METHODS:Sixty NSAA patients were divided equally into two groups.Subjects in the experimental group were treated with Busuishengxue granules,and the control group with Zaizaoshengxue tablets.The treatment course was 6 months and cu-rative efficacy was compared between the two groups as well as with 10 healthy individuals.Flow cytometry(FCM)was used to detect the intracellular concentration of Ca2+([Ca2+]i).Western blotting was employed to detect the expression of enzymes in the MAPK/ERK pathway.RESULTS:The efficacy of Busuishengxue granules was significantly better than that of Zaizaoshengxue tablets(P<0.05).Before treatment,expression of JNK,phospho-ERK 1/2 and p-JNK was higher,and[Ca2+]i higher,than that of the control group(P<0.05).After treatment with Busuishengxue granules,expression of all enzymes related to signal transduction pathways in the blood cells of NSSA patients were altered to different degrees.CONCLUSION:Busuishengxue granules had a better effect with regard to improving symptom scores,increasing the number of blood leukocytes,and increasing hemoglobin levels than Zaizaoshengxue tablets,and they differed slightly in terms of increasing the number of platelets.OBJECTIVE：To observe the clinical efficacy of Busu- ishengxue granules on non-severe aplastic anemia（NSAA） and investigate its effect on the mitogen-ac- tivated protein kinase/extracellular signal-regulat- ed kinase（MAPK/ERK） pathway. METHODS： Sixty NSAA patients were divided equally into two groups. Subjects in the experimen- tal group were treated with Busuishengxue gran- ules, and the control group with Zaizaoshengxue tablets.The treatment course was 6 months and cu-rative efficacy was compared between the two groups as well as with 10 healthy individuals. Flow cytometry（FCM） was used to detect the intracellu- lar concentration of Ca2 ＋（[Ca2 ＋]i）. Western blotting was employed to detect the expression of enzymes in the MAPK/ERK pathway. RESULTS： The efficacy of Busuishengxue granules was significantly better than that of Zaizaoshengx- ue tablets（P0.05）. Before treatment, expression of JNK, phospho-ERK 1/2 and p-JNK was higher, and [Ca2 ＋]i higher, than that of the control group（P 0.05）. After treatment with Busuishengxue gran- ules, expression of all enzymes related to signal transduction pathways in the blood cells of NSSA patients were altered to different degrees. CONCLUSION： Busuishengxue granules had a bet- ter effect with regard to improving symptom scores, increasing the number of blood leukocytes, and increasing hemoglobin levels than Zaizaosh- engxue tablets, and they differed slightly in terms of increasing the number of platelets.

关 键 词：ANEMIA APLASTIC MAP kinase signaling system Busuishengxue granule Zaizaoshengxue tablet 

分 类 号：R556[医药卫生—血液循环系统疾病]