同时测定质控盲样中苯甲酸、山梨酸和糖精钠的高效液相色谱法  

Simultaneous determination of benzoic acid,sorbic acid and saccharin sodium in blind quality control samples by high performance liquid chromatography

作　　者：于浩[1] 曹静[1] 姚协祯 朱丽君[1] 

机构地区：[1]山东省德州市疾病预防控制中心,253016

出　　处：《职业与健康》2014年第3期321-323,共3页Occupation and Health

摘　　要：目的建立一种同时测定质控盲样中苯甲酸、山梨酸、糖精钠的分析方法。方法采用SunFire-C18色谱柱（5μm×4．6mm×150mm），以甲醇＋0．02mol／L乙酸铵（12＋88）作为流动相，高效液相色谱法（HPLC）同时测定质控盲样中3种成分。结果苯甲酸、山梨酸、糖精钠3种成分的分离效果理想，线性关系良好，相关系数均大于0．9999，回收率在96．8％～102．0％之间，相对标准差苯甲酸为3．87％，山梨酸为1．51％，糖精钠为3．30％。结论HPLC法简便、快速，灵敏度高，分离度好，回收率高，结果准确可靠，适用于质控盲样中苯甲酸、山梨酸、糖精钠的同时测定。[ Objective ] To establish a method for simultaneous determination of benzoic acid, sorbic acid and saccharin sodium in blind quality control samples. [ Methods] By using the SunFire C-18 column （5 μm × 4.6 mm × 150 mm）, the mobile phase was methanol ＋ 0.02 mol/L ammonium acetate （ 12 ＋ 88 }, and the contents of three components in blind quality control samples were determined by high performance liquid chromatography （ HPLC }. [ Results ] An ideal separation effect of benzoic acid, sorbic acid and saccharin sodium was achieved, with good linear relationship. The correlation coefficients were greater than 0. 999 9, and the recovery rates were between 96.8% and 102.0%. The relative standard deviation of benzoic acid, sorbic acid and saccharin sodium was 3.87%, 1.51% and 3.30%, respectively. [ Conclusion] HPLC is a simple and rapid method, with high sensitivity, good resolution and high recovery rate, and the results are accurate and reliable. It can be applied to the simultaneous determination of benzoic acid, sorbic acid and saccharin sodium in blind quality control samples.

关 键 词：高效液相色谱法 质控盲样 苯甲酸、山梨酸、糖精钠 

分 类 号：R155.5[医药卫生—营养与食品卫生学]