血管紧张素Ⅱ及氯沙坦对大鼠肾系膜细胞SUMO蛋白表达的影响  

Effects of angiotensinⅡand losartan on expression of SUMO in rat glomerular mesangial cells

作　　者：黄炜[1] 周雪琴[1] 徐玲[1] 徐勇[1] 杨茂君[1] 

机构地区：[1]泸州医学院附属医院内分泌科,四川泸州646000

出　　处：《重庆医学》2014年第3期273-275,共3页Chongqing medicine

基　　金：四川省教育厅科研项目资助(11ZB223)

摘　　要：目的探讨血管紧张素Ⅱ(AngⅡ)及氯沙坦对大鼠肾系膜细胞SUMO蛋白表达的影响。方法将体外培养的大鼠肾系膜细胞分别设正常对照组(NC组)、不同浓度的AngⅡ干预组(A1、A2、A3组)、氯沙坦预处理组(MT组);用Western blot和RT-PCR法检测培养不同时间后各组细胞SUMO1、SUMO2/3蛋白和mRNA表达。结果与NC组比较,AngⅡ干预组和MT组SUMO1、SUMO2/3蛋白和mRNA表达增强(P<0.01);与AngⅡ干预组比较,MT组SUMO1、SUMO2/3蛋白和mRNA表达差异无统计学意义(P>0.05)。结论 AngⅡ呈一定浓度依赖性地上调大鼠肾系膜细胞SUMO蛋白表达,该效应不被氯沙坦所阻断,可能参与糖尿病肾病的发病。Objective To investigate the effects of angiotensin Ⅱ （Ang Ⅱ ） and losartan on the expression of small ubiquitin-related modifier（SUMO） protein （SUMO1 ,SUMO2/3） in cultured rat glomerular mesangial cells（GMCs） .Methods In vitro cul-tured HBZY-1 rat GMCs were divided into 5 groups：normal control group（NC group） ,different concentrations of Ang Ⅱinterven-tion groups（A1 ,A2 ,A3 groups） and losartan treatment group（MT group） .The expression of SUMO1 and SUMO2/3 protein and mRNA of each group was measured by Western blot and RT-PCR .Results Compared with the NC group ,the expression of SU-MO1 and SUMO2/3 protein and mRNA in the Ang Ⅱintervention groups and the losartan treatment group was increased signifi-cantly （P＆lt;0 .01）;Compared with the Ang Ⅱintervention groups ,the expression of SUMO1 and SUMO2/3 protein and mRNA in the losartan treatment group had no statistically significant difference .Conclusion Ang Ⅱ up-regulates the expression of SUMO protein in rGMCs by a dose-dependent manner in certain range ,this effect is not blocked by losartan ,Ang Ⅱ may be involved in the pathogenesis of diabetic nephropathy .

关 键 词：小泛素相关修饰蛋白质类 血管紧张素Ⅱ 氯沙坦 糖尿病肾病 ANGIOTENSIN Ⅱ 

分 类 号：R587.2[医药卫生—内分泌] R692[医药卫生—内科学]