氧诱导支气管肺发育不良鼠肺微血管发育及CD34表达情况  

Pulmonary Microvascular Development and CD34 Expression in Bronchopulmonary Dysplasia Induced by Postnatal Hyperoxia in Mice

作　　者：李秋平[1] 马兴娜[1] 张小英[1] 许靖[1] 章晟[1] 王春枝[1] 封志纯[1] 

机构地区：[1]第二军医大学北京军区总医院临床医学院附属八一儿童医院NICU,北京100700

出　　处：《解放军医药杂志》2014年第1期25-28,39,共5页Medical & Pharmaceutical Journal of Chinese People’s Liberation Army

基　　金：国家自然科学基金青年基金项目(81000265)

摘　　要：目的观察氧诱导支气管肺发育不良(bronchopulmonary dysplasia,BPD)鼠模型肺微血管发育和CD34表达情况。方法采用持续高浓度氧暴露建立致新生小鼠BPD模型,将48只2日龄C57BL/6小鼠,随机分为BPD组和空气组,每组24只,BPD组养殖箱内氧气浓度维持70%±5%,于生后第3、7、14、21天两组分别处死6只取肺组织,观察肺组织结构变化和辐射状肺泡计数(radial alveolar counts,RAC),用免疫组化检测CD34表达水平,image pro plus6.0软件计算微血管密度(MVD)及平均光密度(average optical density,AOD)。结果与空气组比较,BPD组肺组织CD34表达量减少,MVD明显减少,肺泡数量简化。BPD组生后第7、14、21天RAC低于空气组,生后第14、21天MVD和平均AOD均低于空气组(P<0.05)。结论持续高浓度氧吸入可导致新生鼠肺微血管发育异常,MVD减少,血管内皮细胞CD34表达水平降低,可能是BPD发生的重要机制。Objective To observer changes of pulmonary microvascular development and CD34 expression in bronchopulmonary dysplasia （BPD） induced by postnatal hyperoxia in mice. Methods BPD of neonatal mice models were established by exposing in constant hyperxia condition, C57BLl6 48 mice （2 d of birth） were randomly divided into BPD group （n = 24） and air group （n = 24）. The mice in BPD group were exposed in 70% ± 5% oxygen concentration of incubators, and 6 mice in the two groups were respectively killed on 3^st d , 7^th d, 14th d and 21^st d after birth, and then changes of pulmonary tissue structures and radial alveolar counting （RAC） in removed lung tissues were observed; CD34 expressions were detected by immunohistochemical method; microvessel densities （MVD） and average optical density （AOD） were counted by image pro plus 6. 0 software. Results Compared with those of air group, CD34 expressions and MVD were obviously decreased, alveolus numbers were simplified in BPD group. RACs were significantly decreased on 7th d, 14th d and 21 th d after birth in BPD group compared with those of air group, and levels of MVD and AOD on 14th d and 21＂ d after birth were significantly lower than those in air group （P 〈 0.05）. Conclusion Constant high concentration of oxygen inhalation can induce pulmonic microvascular dysplasia, decreased MVD and lower levels of CD34 expression of vascular endothelial cells in neonatal mice, and it may play an important role in the BPD pathogenesis.

关 键 词：支气管肺发育不良 微血管 抗原 CD34 小鼠 

分 类 号：R-332[医药卫生] R722.6