基于脾虚大鼠有机阴离子转运肽2a1动态表达的脾主运化内涵探讨  

Exploration on the nature of spleen govering transportation and transformation based on the dynamic expression of oatp2a1 in spleen-deficiency rats

作　　者：项婷[1] 杨璋斌[2] 孙保国[1] 张诗军[1] 陈泽雄[1] 陈肖霖[1] 吴敏江[1] 

机构地区：[1]中山大学附属第一医院,广州510080 [2]中山大学附属第五医院,珠海519000

出　　处：《中华中医药杂志》2014年第2期430-434,共5页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金(No.81072806);"十一五"国家科技重大专项(No.2009ZX10005-016);广东省自然科学基金(No.S2012010009277)~~

摘　　要：目的:通过观察脾虚大鼠马兜铃酸-Ⅰ(AA-Ⅰ)的代谢与肺、肝、胃、小肠组织有机阴离子转运肽2a1(oatp2a1)表达的关系探讨脾主运化的内涵。方法:SD大鼠随机分为空白组、脾虚组、空白+AA-Ⅰ组、脾虚+AA-Ⅰ组。脾虚组、空白组做AA-Ⅰ药代动力学,并取肺、胃、肝、小肠组织;空白+AA-Ⅰ组、脾虚+AA-Ⅰ组予AA-Ⅰ灌胃后5、60m in分别取上述组织,检测各组织中o a tp2a1表达情况。结果:脾虚组与空白组的AA-Ⅰ代谢出现差异。oatp2a1 mRNA表达:脾虚组中肺、肝、胃组织表达量较空白组升高(P<0.01,P<0.05),空白+AA-Ⅰ组60m in中小肠组织表达量较空白组降低(P<0.01),脾虚+A A-Ⅰ组中5min肺、肝组织表达量较脾虚组降低(P<0.05)。结论:脾虚状态下,AA-Ⅰ代谢差异与肺、肝、胃、小肠组织oatp2a1表达改变相关,提示肝、胃、小肠在脾主运化中发挥重要作用,肺发挥协调作用。Objective： To explore the extensive concept of spleen governing transportation and transformation on the relationship between the expression of oatp2al in lung, liver, stomach and small intestine and the metabolism of AA- I in spleen-deficiency rats. Methods： SD rats were randomly divided into 4 groups： blank group, spleen-deficiency group, blank＋AA- I group and spleen-deficiency＋AA- I group. The pharmacokinetics of AA- I were made and tissues of lung, stomach, liver, small intestine were taken in blank group and spleen-deficiency group; 4 tissues were taken after being given AA- I in 5min and 60min in blank＋AA- I group and spleen-deficiency＋AA- I group. Expression level of the 4 kinds of tissues in each group was detected. Results： The metabolism of AA- I in blank group and spleen-deficiency group was different. The oatp2al mRNA expressed higher in lung, liver and stomach tissues in spleen-deficiency group compared to blank group （P〈0.01, P〈0.05）. The oatp2al mRNA expressed lower in small intestine tissue in blank＋AA- I 60min group compared to blank group （P〈0.01）. The oatp2al mRNA expressed lower in lung and liver tissues in spleen-deficiency＋AA- I 5min group compared to spleen-deficiency group （P〈0.05）. Conclusion： The change of the expression of oatp2al is correlated with the metabolism of AA- I in spleen-deficiency rats which suggests that lung, liver, stomach and small intestine might play a great role in the function of spleen governing transportation and transformation.

关 键 词：有机阴离子转运肽2a1 马兜铃酸 脾虚证 脾主运化 

分 类 号：R228[医药卫生—中医基础理论]