PTTG和VEGF-C在喉癌组织中的表达及其与微淋巴管生成的关系  

The expression of PTTG,VEGF-C and LMVD in laryngeal carcinoma and the relationship among them

作　　者：高珊[1] 卢国友 王竹君[1] 徐勤[1] 辛国华[1] 刘吉娜[1] 黄越燕[1] 

机构地区：[1]承德医学院附属医院耳鼻喉科,河北承德067000 [2]河北省承德市口腔医院综合科,067000

出　　处：《重庆医学》2014年第4期434-436,共3页Chongqing medicine

摘　　要：目的探讨垂体肿瘤转化基因(PTTG)和血管内皮生长因子-C(VEGF-C)在喉癌组织中表达的相关性以及与喉癌发展的关系。方法应用免疫组织化学SP法分别检测60例喉癌组织和32例癌旁组织中PTTG和VEGF-C两种蛋白的表达情况,并用D2-40阳性产物定位淋巴管内皮细胞细胞质及细胞膜,计数微淋巴管密度(LMVD)。结果喉癌组织中PTTG和VEGF-C表达水平均显著高于癌旁组织,差异有统计学意义(P<0.05);二者表达量与喉癌的分化程度、有无淋巴结转移及临床分期差异均有统计学意义(P<0.05),与临床分型、有无吸烟史、肿瘤大小、性别及年龄方面的差异均无统计学意义(P>0.05)。喉癌组织中LMVD表达水平明显高于癌旁组织,差异有统计学意义(P<0.05),与淋巴结转移显著相关(P<0.05),与分化程度、临床分期、临床分型、有无吸烟史、肿瘤大小、性别及年龄不相关(P>0.05)。PTTG和VEGF-C在喉癌组织中表达呈显著正相关(P<0.05)。LMVD分别与喉鳞癌组织中PTTG和VEGF-C蛋白表达正相关(P<0.05)。结论 PTTG和VEGF-C在喉癌的发展中起重要作用,二者的检测可作为喉癌转移及预后的预判指标,有望成为新的基因治疗靶点。Objective To investigate the expression of PTTG and VEGF-C in laryngeal carcinoma and the effect of angiogene-sis .Methods Immunohistochemistry was used to detect the expression of PTTG 、VEGF-C and LMVD in 60 cases of laryngeal car-cinoma and 32 cases of para-carcinoma tissue .D2 40 positive products was used to locate lymphatic endothelial cell cytoplasm and cell membrane ,and count lymphatic microvessel density （LMVD） .Results The expression of PTTG and VEGF-C in laryngeal car-cinoma was significantly higher than that in para-carcinoma tissue（P〈0 .05） .In laryngeal carcinoma ,the expression of PTTG and VEGF-C were associated with differentiation ,lymph node metastasis and clinical stage（P〈0 .05） ,but independent of clinical classi-fication ,smoking history ,tumor′size ,sex and gender（P〉0 .05） .The expression of LMVD in laryngeal carcinoma was significantly higher than that in para-carcinoma tissue（P〈0 .05） .The expression of LMVD was associated with lymph node metastasis （P〈0 .05） ,but independent of differentiation ,clinical classification ,clinical stage smoking history ,tumor′size ,sex and gender （ P〉0 .05） .A significantly positive relation was found between PTTG and VEGF-C（P〈0 .05） .And there were positive relation between PTTG and LMVD、VEGF-C and LMVD（P〈0 .05） .Conclusion PTTG and VEGF-C might play an important role in the carcino-genesis and development of laryngeal carcinoma .PTTG and VEGF-C could be a prognostic factor of colorectal cancer and a new tar-get of gene therapy .

关 键 词：喉肿瘤 微淋巴管密度 垂体肿瘤转化基因 

分 类 号：R739.65[医药卫生—肿瘤]