不同浓度的葡萄糖对SW480肿瘤细胞分泌IGF-1、TGF-β_1的影响  

Effect of different concentrations of glucose on secretions of IGF-1 and TGF-β_1 in SW480 tumor cells

作　　者：张素敏[1] 王彤[2] 刘海东[1] 王祯[1] 许立刚[1] 

机构地区：[1]河北省保定市第三中心医院,河北保定071000 [2]河北大学附属医院,河北保定071000

出　　处：《现代中西医结合杂志》2014年第6期577-579,共3页Modern Journal of Integrated Traditional Chinese and Western Medicine

摘　　要：目的研究不同浓度葡萄糖对SW480肿瘤细胞胰岛素样生长因子1(IGF-1)、转化生长因子β1(TGF-β1)表达的影响,探讨糖尿病发生结直肠癌的相关因素。方法体外常规培养SW480肿瘤细胞。根据所用培养液外加葡萄糖浓度的不同,将实验所用细胞共分6组:0组(对照组)为RPMI1640培养液;1组为RPMI1640培养液+5 mmol/L葡萄糖;2组为RPMI1640培养液+10 mmol/L葡萄糖;3组为RPMI1640培养液+15 mmol/L葡萄糖;4组为RPMI1640培养液+20 mmol/L葡萄糖;5组为RPMI1640培养液+25 mmol/L葡萄糖。每组设3个复孔,置于37℃、5%CO2饱和湿度的培养箱内培养48 h,培养结束后收集细胞培养上清,ELISA法检测IGF-1、TGF-β1的浓度。结果①不同浓度的葡萄糖组IGF-1的表达无显著性差异(F=0.192,P均>0.05)。②4组、5组TGF-β1的浓度明显高于0组,有显著性差异(F=26.036,P均<0.01)。结论葡萄糖不能够上调SW480肿瘤细胞IGF-1的表达,但高浓度的葡萄糖能够上调SW480肿瘤细胞TGF-β1的表达。Objective It is to observe the effect of different concentrations of glucose on the secretions of insulin and insufin-like growth factor - 1 （ IGF - 1 ） and transforming growth factor （ TGF - β1 ） in SW480 tumor cells, and approach the relevant factors of development of colorectal cancer in diabetes mellitus. Methods SW480 tumor cells were cultured in vitro. Ac- cording to the concentrations of glucose in culture medium, the cells were divided into six groups： group 0 with RPMI1640, group 1 with RPMI1640 ＋5 mmol/L glucose, group 2 with RPMI1640 ＋ 10 mmol/L glucose, group 3 with RPMI1640 ＋ 15 mmol/L glucose, group 4 with RPMI1640 ＋ 20 mmol/L glucose, group 5 with RPMI1640 ＋ 25 mmol/L glucose. After added to different culture medium, the cells were cultured 48h, supernatant were collected. The concentrations of IGF- l and TGF- β1 in the supernatant were detected by specific enzyme-linked immunosorbent assay （ELISA）. Results ①There was no signifi- cant difference in IGF - 1 level in SW480 tumor cells among groups of different concentrations of glucose （ all P 〉 0.05 ）. ② The levels of TGF - β1 in group 4 and group 5 was significantly higher than that in group 0 （all P 〈0.01 ）. Conclusion Glu- cose has no effect on the secretion of IGF - 1 in SW480 tumor cells. High concentration of glucose may up-regulate the secretion of TGF - 131 in SW480 tumor cells.

关 键 词：葡萄糖 胰岛素样生长因子1 转化生长因子Β1 糖尿病 结直肠癌 

分 类 号：R735.3[医药卫生—肿瘤]