Isolation and Genetic Analysis of Arabidopsis Mutants with Low_K^+ Tolerance  

拟南芥耐低钾突变体的筛选及遗传分析(英文)

作　　者：赵淑清[1] 林春[1] 杨小贺[1] 武维华[1] 

机构地区：[1]中国农业大学生物学院植物科学系农业部植物生理生化开放实验室,北京100094

出　　处：《Acta Botanica Sinica》2001年第1期105-107,共3页Acta Botanica Sinica（植物学报：英文版）

基　　金：SupportedbytheNationalNaturalScienceFoundationofChina ( 3 95 70 0 74;3 95 2 5 0 0 3 ;and 3 9873 95 6toWWH)andBeijingScienceFou

摘　　要：Ethyl methane-sulfonate (EMS)-mutagenized Arabidopsis M-2 populations were screened in low-K+ medium using the root-bending assay. Forty-two putative low-k(+)-tolerant (lkt) mutants were selected from 150 000 tested M-2 seedlings, and two of these mutants maintained their low-K+-tolerant phenotype in their M-3 generations, respectively. Genetic analysis showed that either one of these two mutants has a monogenic recessive mutation in a nuclear gene, and that the two mutations in two independent mutants are allelic to each other.利用乙酰甲基磺酸 (EMS)诱变方法 ,以幼苗根在重力作用下的弯曲生长为指标 ,筛选得到了拟南芥 (Ara bidopsisthaliana)耐低钾突变体。经过对突变体杂交后代的遗传分析证明 ,其中两株突变体的耐低钾性状为隐性单基因突变所致。鉴定。

关 键 词：Arabidopsis thaliana low K+-tolerance MUTANT 

分 类 号：Q945.7[生物学—植物学]