家蚕肠液蛋白酶消化生理参数的研究  

Study on the digestion physiology parameter of protease from intestine of silkworm

作　　者：程佳[1] 刘朝良[1] 王在贵[1] 张宏福[2] 杨文静[1] 韩薇[1] 程昌粟 项菲菲[1] 

机构地区：[1]安徽农业大学生命科学学院,合肥230036 [2]中国农业科学院北京畜牧兽医研究所动物营养学国家重点实验室,北京100193

出　　处：《安徽农业大学学报》2014年第1期56-59,共4页Journal of Anhui Agricultural University

基　　金：动物营养学国家重点实验室开放基金项目(2004DA125184F1104);转基因专项子项目"猪肽YY(Petide YY)蛋白功能研究";国家星火计划项目(2013GA710092)共同资助

摘　　要：家蚕肠液蛋白酶对家蚕消化吸收蛋白质及其生长发育有着十分重要的作用。为探明影响家蚕肠道蛋白酶活力的因素,从家蚕肠道内容物中分离出蛋白酶酶液,在不同温度、pH及金属离子处理条件下,用福林-酚法测定酶活力变化情况,确定家蚕肠液蛋白酶的最适作用条件,同时研究了家蚕中肠蛋白酶活力随龄期增长的变化规律。结果表明,在35℃、pH10.0左右条件下蛋白酶活力最大,低浓度Mn2+、Fe2+、Cu2+对酶活有抑制作用,高浓度Ca2+对酶有抑制作用,Mg2+浓度对酶活物影响不明显,家蚕中肠蛋白酶活力随龄期增长而增强。Protease in silkworm gut takes an important role in the protein digesting process and development of silkworm. In order to prove the factors influencing the protease activity in silkworm gut, we isolated the prote- ase enzyme from the contents of silkworm gut, and determined the changing process of protease activity under different temperatures, pH values and metal ion concentrations by Folin phenol method, so as to make sure the most suitable conditions for the protease in silkworm gut. At the same time, the change of protease activity in midgut of silkworm with the increase of age was researched. The results indicated that the maximum activity of protease were at 35 ℃, and pH 10.1. The low concentrations of Mn2＋, Fe2＋ and Cu2＋ and the high concentration of Ca2＋ inhibited enzyme activity, while the concentration of Mg2＋ showed significant effect on the enzyme activity. The protease activity in midgut increased greatly with the growth of silkworm.

关 键 词：家蚕 肠道内容物 蛋白酶活性 福林一酚法 

分 类 号：S881.24[农业科学—特种经济动物饲养]