Pseudomonas sp.QJX-1的锰氧化特性研究  

Characterization of Manganese Oxidation by Pseudomonas sp. QJX-1

作　　者：周娜娜[1,2] 柏耀辉 梁金松[2] 罗金明[2] 刘锐平[2] 胡承志[2] 袁林江[1] 

机构地区：[1]西安建筑科技大学环境与市政工程学院,西安710055 [2]中国科学院生态环境研究中心环境水质学国家重点实验室,北京100085

出　　处：《环境科学》2014年第2期740-745,共6页Environmental Science

基　　金：国家自然科学基金项目(41171377);中国科学院知识创新工程重要项目(KZCX2-EW-410)

摘　　要：从锰矿土壤样品中分离、纯化出1株高效锰氧化细菌(QJX-1),经16S rDNA序列鉴定为Pseudomonas sp.QJX-1.研究表明,Pseudomonas sp.QJX-1含有锰氧化的必需成分多铜氧化酶基因CumA,当初始Mn2+为5.05 mg·L-1,菌密度D600为0.020时,该菌可在48 h内将Mn2+转化,且转化率高达99.4%.在寡营养条件下该菌锰氧化速率较富营养条件下有显著提高;添加石英砂滤料促使生物膜的快速形成,进而促进Mn2+的生物转化.根据研究结果推测地下水处理过程中生物锰氧化速率较快.A manganese-oxidizing bacteria （ QJX- 1 ） was isolated from the soil of a manganese mine. It was identified as Pseudomonas sp. QJX-1 by 16S rDNA sequencing. Experimental results showed that the Pseudomonas sp. QJX-1 has a multi-copper oxidase gene CumA, which is an essential component for manganese oxidation by Pseudomonas sp. Under the condition of low initial inoculum level （D600, 0.020）, 5.05 mg·L-1 Mn2＋ could be oxidized by QJX-1 within 48 h with a conversion rate of as high as 99.4%. In comparison with the eutrophic conditions, the oligotrophic condition dramatically increased the biological manganese oxidation rate. Biofilm formation by employing the quartz sand could further improve the oxidation rate of Mn2＋. Based on these results, it is speculated that biological manganese oxidation in underground water treatment is comparatively high.

关 键 词：PSEUDOMONAS SP QJX-1 生物锰氧化 寡营养 石英砂 生物膜 

分 类 号：X172[环境科学与工程—环境科学]