BDE-47降解菌的筛选及破乳提高回收率的方法优化  

Screening of BDE-47 Degrading Bacteria and Optimization of Improving Recovery Rate During the Degradation Process

作　　者：王继华[1] 关健飞[1] 宋北[1] 刘翔[2] 辛佳[2] 

机构地区：[1]哈尔滨师范大学生命科学与技术学院,哈尔滨150025 [2]清华大学环境学院,北京100084

出　　处：《北京工业大学学报》2014年第2期309-314,共6页Journal of Beijing University of Technology

基　　金：国家自然科学基金资助项目(51108145);哈尔滨市科技局青年科技创新人才研究专项基金资助项目(2011RFQXS093);黑龙江省教育厅科学技术研究重点资助项目(12521z010)

摘　　要：通过污染物质量浓度梯度驯化法、最大污染物质量浓度驯化法及外加碳源辅助驯化法,并从整体驯化和分离单菌后驯化2个角度出发,以期系统地筛选出BDE-47高效降解菌种或菌群.测定降解能力过程中,应用物理和化学破乳方法,解决由于细菌表面的疏水性及其分泌的胞外物导致的液液萃取测定回收率过程中的严重乳化问题.结果表明:采用3种方法整体驯化之后进行菌株的分离,分别得到7、3和11株降解菌株,先分离菌株,再采用3种方法逐级驯化共得到能在以BDE-47为唯一碳源的培养基中生长的14株,为驯化最优组合,且经形态学观察及生理生化鉴定可知,先整体驯化再分离得到的菌株,均可在上述14株中找到相对应菌株;水浴、离心及甲醇共同作用的破乳过程为最优破乳组合,其回收率可高达92.3%;甲醇在整个破乳过程中,对BDE-47的回收起积极促进作用,而NaCl则起抑制作用.Three acclimation methods including the gradient pollutant concentration acclimation, maximum contaminant level acclimation and external carbon source assisted acclimation were compared to optimize the screening efficiency of BDE-47 high efficiency degradation strains or flora. In addition, physical and chemical demulsifying methods were used and compared to enhance the recovery rate of liquid-liquid extraction that was negatively affected by emulsification caused by bacterial surface hydrophobicity and secretion of extracellular products. Results showed that a maximum amount of 14 strains that were able to feed on BDE-47 as the sole carbon source were finally obtained when the single strain was first isolated followed with progressive acclimation, while the acclimation and isolation step were sequentially reversed, only 7, 3 and 11 strains were screened, and all of these strains can be found corresponding strains in the 14 strains by morphological observation, respectively. The combination of bath, centrifugal and methanol interaction demulsification process was optimal demulsification, and the recovery rate could be as high as 92.3%. NaC1 throughout the demulsification process for recovery of BDE-47 played a negative role, while the use of methanol played a positive one.

关 键 词：BDE-47 降解菌 驯化 破乳 回收率 

分 类 号：X172[环境科学与工程—环境科学]